Lindner G, Grosse G, Matthies H, Kirsche W
Z Mikrosk Anat Forsch. 1975;89(5):815-23.
Explants of PNS and CNS of chick embryos or fetal rats were cultivated and brainhydrolysat cerebrolysin or a synthetic aminoacidsolution or a brain extrakt was applied to the cultures. Cerebrolysin exercised in vitro an effect, which is related to charge only trifling. The cerebrolysin effect is essential related to the concentration/medium. High concentration of cerebrolysin decreases the growth of nerve fibers. Migration of non-neuronal cells increases. A stimulation of nerve fibers growth resembling to NGF was not seen. The density of cells or number of cells/volume unit, rate of mitosis and phases of mitotic non-neuronal cells is changed by cerebrolysin. The effect of cerebrolysin in vitro is compared to the tested aminoacidsolution and brainextract resembling. NGF may be important selectiv for stimulation of the activity of sympathetic and sensoric ganglia. Brainextract and brainhydrolysat and aminoacidsolution influenced in vitro PNS- and CNS-tissue cultures.
培养鸡胚或胎鼠的周围神经系统（PNS）和中枢神经系统（CNS）外植体，并将脑水解物脑蛋白水解物或合成氨基酸溶液或脑提取物应用于培养物中。脑蛋白水解物在体外产生的效应与电荷仅存在轻微关联。脑蛋白水解物的效应与浓度/培养基密切相关。高浓度的脑蛋白水解物会降低神经纤维的生长。非神经元细胞的迁移增加。未观察到类似神经生长因子（NGF）对神经纤维生长的刺激作用。脑蛋白水解物会改变细胞密度或单位体积内的细胞数量、有丝分裂率以及有丝分裂非神经元细胞的阶段。将脑蛋白水解物在体外的效应与测试的氨基酸溶液和脑提取物的效应进行了比较。神经生长因子可能对刺激交感神经节和感觉神经节的活性具有重要的选择性。脑提取物、脑水解物和氨基酸溶液在体外对周围神经系统和中枢神经系统组织培养物产生了影响。
