Grosse G, Lindner G
Z Mikrosk Anat Forsch. 1981;95(2):191-6.
Explants of nervous tissue (CNS: hippocampus from fetal rats, telencephalon and PNS: ganglion trigeminale from chick embryos) were cultivated in maximow chambers. Biological extracts (brain and embryo extract) or chemical agents (aminoacid mixtures and peptides with known sequence of amino acids) were tested. Biological extracts and aminoacid mixtures stimulated the differentiation in PNS and CNS cultures. A stimulating effect of peptides seems to exist only on PNS explants. The fundamental importance of suitable reference systems, parameters, optimal concentrations and periods of application of effective substances for the evaluation of the results is discussed.
将神经组织外植体（中枢神经系统：来自胎鼠的海马体、端脑；外周神经系统：来自鸡胚的三叉神经节）培养于马克西莫夫培养室中。对生物提取物（脑提取物和胚胎提取物）或化学试剂（氨基酸混合物以及具有已知氨基酸序列的肽）进行了测试。生物提取物和氨基酸混合物刺激了外周神经系统和中枢神经系统培养物的分化。肽的刺激作用似乎仅对外周神经系统外植体存在。讨论了合适的参照系统、参数、有效物质的最佳浓度及应用时间对于评估结果的至关重要性。
