Zhu A
The Lindsley F. Kimball Research Institute, The New York Blood Center, New York 10021, USA.
Transplantation. 2000 Jun 15;69(11):2422-8. doi: 10.1097/00007890-200006150-00036.
Xenotransplantation is considered one of possible solutions for the serious shortage of organs and cells in transplantation. Although the alphaGal epitope (Gal alpha1,3Gal beta1,4GlcNAc-R) has been identified as being a major xenoantigen responsible for hyperacute rejection, the removal of anti-alphaGal antibody alone from human serum is insufficient to circumvent antibody-mediated immune responses.
We report here the characterization of xenoreactive human natural antibodies against antigens without the alphaGal epitope (nonalphaGal xenoantigens) on porcine erythrocytes using flow cytometry and the evidence for their involvement in complement-mediated hemolysis. Furthermore, a novel protein of 45-kDa has been isolated from the porcine erythrocyte membrane as a major protein antigen recognized by human anti-nonalphaGal.
The data presented here will add to our knowledge of xenoantigens on porcine red cells and be important for developing strategies to produce modified red cells immunologically compatible to humans.
异种移植被认为是解决移植中器官和细胞严重短缺问题的可能方案之一。尽管α-半乳糖表位（Galα1,3Galβ1,4GlcNAc-R）已被确定为导致超急性排斥反应的主要异种抗原，但仅从人血清中去除抗α-半乳糖抗体不足以规避抗体介导的免疫反应。
我们在此报告使用流式细胞术对猪红细胞上无α-半乳糖表位的抗原（非α-半乳糖异种抗原）的异种反应性人类天然抗体进行的表征，以及它们参与补体介导的溶血的证据。此外，已从猪红细胞膜中分离出一种45 kDa的新型蛋白质，作为人类抗非α-半乳糖识别的主要蛋白质抗原。
此处提供的数据将增加我们对猪红细胞异种抗原的了解，并对制定生产与人类免疫相容的修饰红细胞的策略具有重要意义。
