使用离体青蛙脊髓评估人和动物血清中的神经电阻断因子。
Neuroelectric blocking factors in human and animal sera evaluated using the isolated frog spinal cord.
作者信息
Schauf C L, Davis F A, Sack D A, Reed B J, Kesler R L
出版信息
J Neurol Neurosurg Psychiatry. 1976 Jul;39(7):680-5. doi: 10.1136/jnnp.39.7.680.
The effects of sera from guinea-pigs with experimental allergic encephalomyelitis (EAE) and multiple sclerosis (MS) patients were evaluated and compared with the activity of control sera using the isolated frog spinal cord. Ventral root responses (VRR) were recorded during supramaximal ipsilateral dorsal root stimulation in the presence and absence of 25% serum. In control experiments with normal human and guinea-pig sera we observed a consistent, reversible increase in VRR averaging 20% and 17% respectively, and in no case was any significant decrease produced. In contrast, sera from EAE guinea-pigs 12 to 19 days after injection produced an equally rapid, reversible decrease in VRR. The decrease averaged 36% and was highly significant (P less than 0.0001) relative to controls. Similarly, sera from MS patients on the average decreased the VRR by 26%, and this again was significant compared with controls (P less than 10(-6).
利用分离的蛙脊髓，评估并比较了患有实验性变应性脑脊髓炎（EAE）的豚鼠血清和多发性硬化症（MS）患者血清的作用，并与对照血清的活性进行了比较。在存在和不存在25%血清的情况下，在同侧背根超强刺激期间记录腹根反应（VRR）。在使用正常人和豚鼠血清的对照实验中，我们观察到VRR持续、可逆地增加，平均分别为20%和17%，并且在任何情况下都没有产生任何显著的降低。相比之下，注射后12至19天的EAE豚鼠血清使VRR同样迅速、可逆地降低。相对于对照，降低平均为36%，且具有高度显著性（P小于0.0001）。同样，MS患者的血清平均使VRR降低了26%，与对照相比，这再次具有显著性（P小于10⁻⁶）。
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