Howard J G, Courtenay B M, Hale C
Eur J Immunol. 1976 Nov;6(11):837-9. doi: 10.1002/eji.1830061118.
Adult CBA mice were made tolerant with 1 mg levan and tested subsequently for sponteneous recovery by plaque-forming cell assay in response to immunizing challenge. Weakening of tolerance was first detectable only after 100 days, while return to full normal responsiveness required 400 days. Thymectomy 2 weeks prior to tolerization neither retarded nor diminished the recovery process. Loss of tolerance which had been induced by 1 mg dextran B512 was much slower in onset (greater than 250 days), yet not delayed by thymectomy (full recovery was not followed). These findings in relation to other considerations imply that associative recognition by T cells is not required for B cell responses to these "thymus-independent" antigens.
成年CBA小鼠用1毫克左聚糖使其产生耐受性，随后通过空斑形成细胞试验检测其对免疫攻击的自发恢复情况。耐受性减弱仅在100天后才首次可检测到，而恢复到完全正常反应性则需要400天。在耐受诱导前2周进行胸腺切除，既不延迟也不减少恢复过程。由1毫克葡聚糖B512诱导的耐受性丧失开始得要慢得多（超过250天），但胸腺切除并未使其延迟（未观察到完全恢复）。这些发现与其他考虑因素表明，B细胞对这些“胸腺非依赖性”抗原的反应不需要T细胞的联合识别。
