Novogrodsky A, Stenzel K H, Rubin A L
J Immunol. 1977 Mar;118(3):852-7.
Blastogenic responses of normal human peripheral lymphocytes to three distinct groups of mitogens were studied: Group I--phytohemagglutinin (PHA), concanavalin A (Con A), and pokeweed mitogen (PWM); Group II--soybean agglutinin (SBA) and peanut agglutinin (PNA); and Group III--galactose oxidase (GO) and sodium periodate (IO4-). SBA was mitogenic for human cells, and this effect was enhanced by treating the cells with neuraminidase (NA). PNA was mitogenic only after cells had been treated with NA. GO was effective before and activity was increased after lymphocytes were treated with NA. Responses to Group II and III mitogens were more variable than were those to Group I mitogens. Studies with purified T and B cells indicated that SBA and PNA were T cell mitogens, whereas IO4- and GO failed to stimulate either T or B cells. Adding macrophages back to this system indicated that they were both T cell mitogens with strict macrophage requirements. T cell responses to SBA and PNA were enhanced over responses to unfractionated cells to a degree that could not be explained simply by enrichment of the cultures with T cells. Removal of adherent cells from unfractionated cell suspensions again revealed a marked enhancement of responses to SBA and PNA, a consistent decrease in responses to IO4-, and a variable decrease in responses to GO. Similar results were found with 14C-leucine and 3H-uridine incorporation, as well as 3H-thymidine for the assessment of bastogenic response. Mechanisms responsible for these differential effects of macrophage depletion on lymphocyte responses to different groups of mitogens are yet to be determined. Either different mitogens require different lymphocyte to macrophage ratios for optimal stimulation, or some mitogens (i.e., SBA and PNA) form inhibitory complexees in the lymphocyte-macrophage mixture. In any case, variability in response to mitogenic agents in normal as well as pathologic states may be dependent on adherent cell populations, rather than on the lymphocytes themselves.
第一组——植物血凝素（PHA）、刀豆球蛋白A（Con A）和商陆有丝分裂原（PWM）；第二组——大豆凝集素（SBA）和花生凝集素（PNA）；第三组——半乳糖氧化酶（GO）和高碘酸钠（IO4-）。SBA对人细胞有促有丝分裂作用，用神经氨酸酶（NA）处理细胞可增强这种作用。PNA只有在细胞用NA处理后才有促有丝分裂作用。GO在淋巴细胞用NA处理之前有效，处理后活性增加。对第二组和第三组有丝分裂原的反应比第一组有丝分裂原的反应更具变异性。对纯化的T细胞和B细胞的研究表明，SBA和PNA是T细胞有丝分裂原，而IO4-和GO不能刺激T细胞或B细胞。将巨噬细胞重新加入该系统表明，它们都是有严格巨噬细胞需求的T细胞有丝分裂原。T细胞对SBA和PNA的反应比对未分离细胞的反应增强，增强程度不能简单地用培养物中T细胞的富集来解释。从未分离的细胞悬液中去除贴壁细胞再次显示，对SBA和PNA的反应明显增强，对IO4-的反应持续下降，对GO的反应可变下降。用14C-亮氨酸、3H-尿苷掺入以及3H-胸腺嘧啶核苷评估增殖反应时也发现了类似结果。巨噬细胞耗竭对淋巴细胞对不同组有丝分裂原反应的这些差异效应的机制尚待确定。要么不同的有丝分裂原需要不同的淋巴细胞与巨噬细胞比例来实现最佳刺激，要么某些有丝分裂原（即SBA和PNA）在淋巴细胞-巨噬细胞混合物中形成抑制复合物。无论如何，正常和病理状态下对有丝分裂原的反应变异性可能取决于贴壁细胞群体，而不是淋巴细胞本身。
