Fernandes O de F, Costa T R, Costa M R, Soares A J, Pereira A J, Silva M do R
Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO.
Rev Soc Bras Med Trop. 2000 Jan-Feb;33(1):75-8. doi: 10.1590/s0037-86822000000100011.
Cryptococcosis is considered the most frequent fungal systemic opportunist infection in patients with AIDS. C. neoformans var. neoformans infects the patients with AIDS more often than C. neoformans var. gattii which has been rarely isolated from patients with AIDS. Even in endemic regions of C. neoformans var. gattii, the variety of neoformans is still the most common agent diagnosed in cryptococcosis of patients with AIDS. Cerebrospinal fluid (CSF) from fifty patients with neurocryptococcosis associated with AIDS were studied. The fungi were isolated in agar Sabouraud medium, and were identified by microscopic and macroscopic examination by agar Sabouraud, agar niger and Christensen's urea medium. The variety was determined based on a color characteristic reaction on glycine-L-canavanina-bromothymol blue agar. From all of the fifty patients' cerebrospinal fluid (CSF) we were able to recover C. neoformans. The isolates were identified as C. neoformans var. neoformans in 47 patients and as C. neoformans var. gattii. in the 3 remaining patients. These findings suggest that both varieties, gattii and neoformans, are agents of neurocryptococcosis in patients with AIDS.
隐球菌病被认为是艾滋病患者中最常见的真菌性全身机会性感染。新型隐球菌新生变种比格特变种更常感染艾滋病患者，格特变种很少从艾滋病患者中分离出来。即使在新型隐球菌格特变种的流行地区，新生变种仍是艾滋病患者隐球菌病中最常见的病原体。对50例与艾滋病相关的神经隐球菌病患者的脑脊液进行了研究。真菌在沙保弱琼脂培养基中分离，并通过沙保弱琼脂、黑琼脂和克里斯滕森尿素培养基进行显微镜和宏观检查鉴定。根据在甘氨酸-L-刀豆氨酸-溴百里酚蓝琼脂上的颜色特征反应确定变种。从所有50例患者的脑脊液中均分离出新型隐球菌。47例患者的分离株鉴定为新型隐球菌新生变种，其余3例患者的分离株鉴定为新型隐球菌格特变种。这些发现表明，格特变种和新生变种都是艾滋病患者神经隐球菌病的病原体。
