Pérez C, Dolande M, Moya M, Roselló A, de Capriles Claudia R Hartung, Landaeta M E, Mata-Essayag S
Sección de Micología Médica, Instituto de Medicina Tropical, Universidad Central de Venezuela, Apartado 47408, Los Chaguaramos, Caracas 1041, Venezuela.
Mycopathologia. 2008 Sep;166(3):149-53. doi: 10.1007/s11046-008-9132-1. Epub 2008 May 30.
Cryptococcus neoformans is one of the medically important yeast-like fungi. C. neoformans var. gatti has been made a species: C. gatti. In our country, there are few studies about these two species and their serotypes. The aim of this study was to determine the distribution of C. neoformans and C. gattii, and their serotypes in Venezuelan clinical isolates. One hundred and twenty C. neoformans and 12 C. gattii clinical isolates were identified by L-canavanine, glycine, and bromothymol blue agar media (CGB). These were investigated by agglutination and adsorption studies with anticryptococcal sera, which were produced by rabbit immunization. Of the 132 isolates 59.8% were typed serotype A (C. neoformans), followed by 25.8% serotype D (C. neoformans), 5.3% serotype AD (C. neoformans), and 5.3% were typed serotype C (var. gattii). Additionally 3.8% were serotype B (C. gattii).
新型隐球菌是一种具有重要医学意义的酵母样真菌。新型隐球菌格特变种已被定为一个种：格特隐球菌。在我国，关于这两个种及其血清型的研究较少。本研究的目的是确定新型隐球菌和格特隐球菌及其血清型在委内瑞拉临床分离株中的分布情况。通过L-刀豆氨酸、甘氨酸和溴百里酚蓝琼脂培养基（CGB）鉴定了120株新型隐球菌和12株格特隐球菌临床分离株。通过用兔免疫产生的抗隐球菌血清进行凝集和吸附研究对这些菌株进行了调查。在132株分离株中，59.8%为血清型A（新型隐球菌），其次是25.8%为血清型D（新型隐球菌），5.3%为血清型AD（新型隐球菌），5.3%为血清型C（格特变种）。另外，3.8%为血清型B（格特隐球菌）。
