Yin-Murphy M
Bull World Health Organ. 1976;54(6):675-9.
Simple tests for the study of picornavirus epidemic conjunctivitis are described. The virus was successfully isolated in wells of microtitration plates containing HeLa cell suspension and the isolates were easily identifiable by neutralization in the micrometabolic inhibition test. For the estimation of antibody titre in patients' sera, the latter procedure was found to be as reliable as neutralization in tissue culture tubes. These micromethods would enable virus laboratories not equipped for tissue culture work to study this new ocular disease, which has been endemic in a number of countries since the pandemic outbreak of 1969-72 in Africa, Asia, and Europe (London).
本文描述了用于研究微小核糖核酸病毒流行性结膜炎的简易检测方法。该病毒在含有HeLa细胞悬液的微量滴定板孔中成功分离，且分离株在微量代谢抑制试验中通过中和反应易于识别。对于患者血清中抗体滴度的测定，发现后一种方法与在组织培养管中进行的中和反应一样可靠。这些微量方法将使没有配备组织培养工作设施的病毒实验室能够研究这种自1969 - 1972年在非洲、亚洲和欧洲（伦敦）大流行以来在许多国家呈地方流行的新型眼部疾病。
