Yin-Murphy M, Abdul Rahim N, Phoon M C, Howe J
Bull World Health Organ. 1985;63(4):705-9.
Picornavirus particles and serotype-specific neutralizing antibody were demonstrated in tears collected during early onset of acute haemorrhagic conjunctivitis. Virus particles deposited from tears by airfuge ultracentrifugation and stained with potassium phosphotungstate were easily recognized by electron microscopy. Tear neutralizing antibody in the ultracentrifuged supernatant was detected by the neutralization test in monolayer HeLa cells grown in microtitration plates. The presence of virus particles and specific neutralizing antibody in tear specimens correlated with the serological findings.
在急性出血性结膜炎发病初期采集的泪液中发现了微小核糖核酸病毒颗粒和血清型特异性中和抗体。通过空气离心机超速离心从泪液中沉淀出病毒颗粒，并用磷钨酸钾染色，通过电子显微镜很容易识别。通过在微量滴定板中培养的单层HeLa细胞进行中和试验，检测超速离心上清液中的泪液中和抗体。泪液标本中病毒颗粒和特异性中和抗体的存在与血清学结果相关。
