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高效液相色谱法测定12个墨西哥商业马铃薯品种中的糖苷生物碱α-茄碱和α-查茄碱

High-performance liquid chromatographic determination of the glycoalkaloids alpha-solanine and alpha-chaconine in 12 commercial varieties of Mexican potato.

作者信息

Sotelo A, Serrano B

机构信息

Departamento de Farmacia, Universidad Nacional Autónoma de México (UNAM), Cd. Universitaria, México D.F.

出版信息

J Agric Food Chem. 2000 Jun;48(6):2472-5. doi: 10.1021/jf990755t.

DOI:10.1021/jf990755t
PMID:10888570
Abstract

The glycoalkaloid content in 12 commercial varieties of Mexican potatoes was measured by HPLC in both the peel and the flesh of the potato. The principal glycoalkaloids alpha-solanine and alpha-chaconine were present in higher concentration in the peel than in the flesh of all varieties. The main alkaloid in the peel of the potatoes was alpha-chaconine and comprised about 65-71% of the total glycoalkaloids. The high concentration of alpha-chaconine in peel, which is more toxic than alpha-solanine, gives more protection to the tuber against predators. The total alkaloids in the peel of Alpha, Juanita, Michoacan, Norteña, Rosita, and Tollocan varieties were higher than the limit recommended for food safety. However, the peel represents less than 10% of the total tuber in most of the varieties. The total alkaloids contained in the peel of Atzimba, Lopez, Marciana, Montsama, Murca, and Puebla was lower than the limits recommended for food safety. The glycoalkaloid content in the boiled peeled potatoes was less than 9 mg/100 g but in Alpha, Montsama, and Puebla varieties, both glycoalkaloids were absent. According to the results, the consumption of the 12 commercial varieties of Mexican potatoes does not represent any danger to human health.

摘要

通过高效液相色谱法(HPLC)测定了12个墨西哥商业马铃薯品种的薯皮和薯肉中的糖苷生物碱含量。在所有品种中,主要的糖苷生物碱α-茄碱和α-查茄碱在薯皮中的浓度高于薯肉。马铃薯薯皮中的主要生物碱是α-查茄碱,约占总糖苷生物碱的65%-71%。薯皮中α-查茄碱浓度较高,其毒性比α-茄碱更大,能为块茎提供更多抵御捕食者的保护。阿尔法、胡安妮塔、米却肯、诺特尼亚、罗西塔和托洛坎品种薯皮中的总生物碱含量高于食品安全推荐限值。然而,在大多数品种中,薯皮占整个块茎的比例不到10%。阿齐姆巴、洛佩斯、马尔恰纳、蒙萨马、穆尔卡和普埃布拉品种薯皮中的总生物碱含量低于食品安全推荐限值。煮熟去皮马铃薯中的糖苷生物碱含量低于9毫克/100克,但在阿尔法、蒙萨马和普埃布拉品种中,两种糖苷生物碱均未检出。根据结果,食用这12个墨西哥商业马铃薯品种对人体健康不构成任何危险。

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