Tomita M, Matsuzaki Y, Onitsuka T
Department of Surgery II, Miyazaki Medical College, Kiyotake, Japan.
Ann Thorac Surg. 2000 Jun;69(6):1686-90. doi: 10.1016/s0003-4975(00)01160-7.
We conducted a retrospective study to clarify the effect of mast cells on tumor angiogenesis in lung cancer patients.
Formalin-fixed and paraffin-embedded tumor sections were used in this study. Parenchymal mast cells were stained with Alcian blue and safranin O. The number of mast cells per ten fields at a magnification of 200x was counted under light microscopy, and the average count was determined. To highlight the microvessels, endothelial cells were stained with anti-human factor VIII antibody. After the microvessel count was determined, the microvessels were further stained with Alcian blue and safranin O to show areas of mast cell infiltration. Expression of vascular endothelial growth factor was assessed using a polyclonal antibody.
We found a significant correlation between mast cell count and microvessel density. This correlation was also observed in patients with adenocarcinoma (p < 0.001) as well as in patients with squamous cell carcinoma (p < 0.01). Double staining of the microvessels showed highly angiogenic areas densely populated with mast cells. Although we detected a slight trend toward a correlation between vascular endothelial growth factor expression and microvessel density, it was not statistically significant. We found no association between vascular endothelial growth factor expression and mast cell count.
There appears to be a direct correlation between the number of mast cells and tumor angiogenesis in patients with lung cancer, and this relationship appears to be independent of vascular endothelial growth factor expression.
我们进行了一项回顾性研究，以阐明肥大细胞对肺癌患者肿瘤血管生成的影响。
本研究使用福尔马林固定、石蜡包埋的肿瘤切片。实质肥大细胞用阿尔辛蓝和番红O染色。在光学显微镜下以200倍放大倍数计数每十个视野中的肥大细胞数量，并确定平均计数。为突出微血管，内皮细胞用抗人因子VIII抗体染色。确定微血管计数后，微血管进一步用阿尔辛蓝和番红O染色以显示肥大细胞浸润区域。使用多克隆抗体评估血管内皮生长因子的表达。
我们发现肥大细胞计数与微血管密度之间存在显著相关性。在腺癌患者（p < 0.001）和鳞状细胞癌患者（p < 0.01）中也观察到这种相关性。微血管的双重染色显示高度血管生成区域有密集的肥大细胞。尽管我们检测到血管内皮生长因子表达与微血管密度之间有轻微的相关性趋势，但无统计学意义。我们未发现血管内皮生长因子表达与肥大细胞计数之间存在关联。
肺癌患者肥大细胞数量与肿瘤血管生成之间似乎存在直接相关性，且这种关系似乎独立于血管内皮生长因子表达。
