利用小型培养中的Y-1肾上腺细胞检测产肠毒素大肠杆菌
Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture.
作者信息
Sack D A, Sack R B
出版信息
Infect Immun. 1975 Feb;11(2):334-6. doi: 10.1128/iai.11.2.334-336.1975.
Abstract
A rapid, potentially clinically useful test for detection of enterotoxigenic Escherichia coli is described. Whole bacterial cultures of enterotoxigenic E. coli, when briefly exposed to Y1 adrenal cells in tissue miniculture, effect a rounding response in the tissue culture that can be discerned at 18 to 24 h. The tissue culture technique agreed with the rabbit ileal loop in all 58 enterotoxigenic and 52 non-enterotoxigenic E. coli strains tested.
摘要
本文描述了一种快速、可能具有临床实用价值的检测产肠毒素大肠杆菌的试验。产肠毒素大肠杆菌的全菌培养物在组织微量培养中短暂暴露于Y1肾上腺细胞时，可在18至24小时内使组织培养物出现变圆反应。在检测的58株产肠毒素大肠杆菌菌株和52株非产肠毒素大肠杆菌菌株中，该组织培养技术与兔回肠袢试验结果一致。
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本文引用的文献
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Stimulation of steroidogenesis in tissue culture by enterotoxigenic Escherichia coli and its neutralization by specific antiserum.产肠毒素大肠杆菌对组织培养中类固醇生成的刺激作用及其被特异性抗血清的中和作用。
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6
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