产肠毒素大肠杆菌的快速筛查方法。
Rapid screening method for enterotoxigenic Escherichia coli.
作者信息
Gurwith M
出版信息
J Clin Microbiol. 1977 Sep;6(3):314-6. doi: 10.1128/jcm.6.3.314-316.1977.
A rapid screening method for detection of Escherichia coli producing heat-labile enterotoxin is described. Single colonies are transferred directly from primary culture plates into 96-well microculture plates containing 0.3 ml of brain heart infusion broth in each well. After 24 h at 37 degrees C, each brain heart infusion broth culture is assayed by the miniculture method in the corresponding well of a microculture plate in which Y-1 mouse adrenal tumor cells have been grown. All enterotoxigenic isolates detected by this method were confirmed in the assay but with culture supernatants.
本文描述了一种用于检测产热不稳定肠毒素大肠杆菌的快速筛选方法。单菌落直接从原代培养平板转移至96孔微量培养板中，每孔含有0.3 ml脑心浸液肉汤。在37℃培养24小时后，将每个脑心浸液肉汤培养物在已培养Y-1小鼠肾上腺肿瘤细胞的微量培养板的相应孔中通过微量培养法进行检测。通过该方法检测到的所有产肠毒素分离株均在检测中得到证实，但使用的是培养上清液。
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