Sack D A, McLaughlin J C, Sack R B, Orskov F, Orskov I
J Infect Dis. 1977 Feb;135(2):275-80. doi: 10.1093/infdis/135.2.275.
Enterotoxigenic strains of Escherichia coli, a significant cause of acute, watery, cholera-like diarrhea, were isolated from 23 of 65 patients with diarrhea in whom no other enteric pathogens were found during one week (November 1974) at the Cholera Research Hospital, Dacca, Bangladesh. Diarrhea associated with enterotoxigenic strains of E. coli occurred primarily in adults but affected persons of all age groups and could not be distinguished from cholera or from other cholera-like diarrhea on clinical grounds. Routine bacteriologic methods were inadequate for identification of enterotoxigenic E. coli as the etiologic agent of the diarrhea. The enterotoxigenic E. coli, producing heat-stable and/or heat-labile enterotoxin, were detected by use of assays in the Y1 adrenal cell, the Chinese hamster ovary cell, the rabbit ileal loop, and the infant mouse. The two tissue culture assays yielded comparable results in tests with 640 (193 positive, 447 negative) of 643 isolates of E. coli. The results of this study support the idea that enterotoxigenic E. coli play a significant role as pathogens in the etiology of acute watery diarrhea.
产肠毒素大肠杆菌是急性水样霍乱样腹泻的一个重要病因，1974年11月在孟加拉国达卡霍乱研究医院，从65例腹泻患者中的23例分离出了这种病菌，这些患者在一周内未发现其他肠道病原体。与产肠毒素大肠杆菌相关的腹泻主要发生在成年人中，但各年龄组的人都可能受影响，从临床角度无法将其与霍乱或其他霍乱样腹泻区分开来。常规细菌学方法不足以鉴定产肠毒素大肠杆菌作为腹泻的病原体。通过在Y1肾上腺细胞、中国仓鼠卵巢细胞、兔回肠袢和幼鼠中进行检测，发现了产生热稳定和/或热不稳定肠毒素的产肠毒素大肠杆菌。在对643株大肠杆菌分离株中的640株（193株阳性，447株阴性）进行检测时，两种组织培养检测方法得出了可比的结果。这项研究的结果支持了产肠毒素大肠杆菌在急性水样腹泻病因中作为病原体发挥重要作用这一观点。
