酿酒酵母中一般氨基酸通透酶突变体的正向选择
Positive selection of general amino acid permease mutants in Saccharomyces cerevisiae.
作者信息
Rytka J
出版信息
J Bacteriol. 1975 Feb;121(2):562-70. doi: 10.1128/jb.121.2.562-570.1975.
Abstract
It was found that D-stereoisomers of natural amino acids inhibit the growth of Saccharomyces cerevisiae cells. Kinetic and genetic evidence showed that d-amino acids enter the cell by the general amino acid permease. Two classes of S. cerevisiae mutants resistant to d-amino acids were isolated. One class of mutants appeared to be defective in the general amino acid permease specified by the gene gap. In the second class, the activity of general amino acid permease was affected by ammonium ions. Mutants of the second class were isolated in a yeast strain with the general amino acid permease insensitive to the presence of ammonium ions in culture media. The mutation affecting the permease, amc, occurred in a locus unlinked to gap.
摘要
研究发现，天然氨基酸的D-立体异构体可抑制酿酒酵母细胞的生长。动力学和遗传学证据表明，D-氨基酸通过通用氨基酸通透酶进入细胞。分离出了两类对D-氨基酸具有抗性的酿酒酵母突变体。一类突变体似乎在由基因gap指定的通用氨基酸通透酶中存在缺陷。在第二类中，通用氨基酸通透酶的活性受铵离子影响。第二类突变体是在一种通用氨基酸通透酶对培养基中铵离子的存在不敏感的酵母菌株中分离得到的。影响通透酶的突变amc发生在一个与gap不连锁的基因座上。
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本文引用的文献
1
Chromosome Mapping in Saccharomyces: Centromere-Linked Genes.酿酒酵母中的染色体图谱：着丝粒连锁基因。
Genetics. 1960 Aug;45(8):1085-110. doi: 10.1093/genetics/45.8.1085.
2
AMINO ACID ACCUMULATION IN ETHIONINE-RESISTANT SACCHAROMYCES CEREVISIAE.抗乙硫氨酸酿酒酵母中的氨基酸积累
J Bacteriol. 1964 Jul;88(1):20-4. doi: 10.1128/jb.88.1.20-24.1964.
3
[Incorporation of endogenous and exogenous amino acids into proteins of yeast].[内源性和外源性氨基酸掺入酵母蛋白质的过程]
Ann Inst Pasteur (Paris). 1958 Jul;95(1):73-87.
4
Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. 3. Evidence for a specific methionine-transporting system.酿酒酵母中氨基酸通透酶的多样性。3. 特定甲硫氨酸转运系统的证据。
Biochim Biophys Acta. 1967 Jul 3;135(3):507-16. doi: 10.1016/0005-2736(67)90040-5.
5
Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. II. Evidence for a specific lysine-transporting system.酿酒酵母中氨基酸通透酶的多样性。II. 存在特定赖氨酸转运系统的证据。
Biochim Biophys Acta. 1966 Oct 31;127(2):339-46. doi: 10.1016/0304-4165(66)90388-6.
6
Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. I. Evidence for a specific arginine-transporting system.酿酒酵母中氨基酸通透酶的多样性。I. 特定精氨酸转运系统的证据。
Biochim Biophys Acta. 1966 Oct 31;127(2):325-38. doi: 10.1016/0304-4165(66)90387-4.
7
[Properties and genetic control of the system for accumulation of amino acids in Saccharomyces cerevisiae].[酿酒酵母中氨基酸积累系统的特性及遗传控制]
Biochim Biophys Acta. 1965 Oct 18;107(3):546-66.
8
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9
[Purification and properties of acetyl-CoA: D-amino acid-alpha-N-acetyltransferase from yeast].
Biochem Z. 1965 Jun 3;342(1):54-65.
10
Derepression of a proline transport system in Saccharomyces chevalieri by nitrogen starvation.氮饥饿诱导解脂耶氏酵母中脯氨酸转运系统的去阻遏作用
Biochim Biophys Acta. 1969 Mar 11;173(2):302-12. doi: 10.1016/0005-2736(69)90113-8.
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