酿酒酵母通用氨基酸通透酶的转录和翻译后调控
Transcriptional and posttranslational regulation of the general amino acid permease of Saccharomyces cerevisiae.
作者信息
Stanbrough M, Magasanik B
机构信息
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
出版信息
J Bacteriol. 1995 Jan;177(1):94-102. doi: 10.1128/jb.177.1.94-102.1995.
Abstract
The cellular level and activity of the general amino acid permease, the product of the GAP1 gene of Saccharomyces cerevisiae, are regulated at the level of transcription by two systems, the products of URE2/GLN3 and NIL1 in response to the nitrogen sources of the growth medium and inactivation in response to the presence of glutamine or glutamate. Active permease is phosphorylated. The addition of glutamine causes rapid dephosphorylation and inactivation of the permease with the same kinetics, which is followed by slower disappearance of the protein. These results suggest that inactivation of the permease results from its dephosphorylation.
摘要
酿酒酵母GAP1基因的产物——通用氨基酸通透酶的细胞水平和活性，在转录水平受到两个系统的调控，即URE2/GLN3和NIL1的产物，它们响应生长培养基中的氮源，并且在谷氨酰胺或谷氨酸存在时发生失活。活性通透酶被磷酸化。添加谷氨酰胺会导致通透酶快速去磷酸化并失活，其动力学相同，随后蛋白质会较慢消失。这些结果表明通透酶的失活是由其去磷酸化导致的。
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