酿酒酵母中的脯氨酸转运
Proline transport in Saccharomyces cerevisiae.
作者信息
Lasko P F, Brandriss M C
出版信息
J Bacteriol. 1981 Oct;148(1):241-7. doi: 10.1128/jb.148.1.241-247.1981.
Abstract
The yeast Saccharomyces cerevisiae is capable of utilizing proline as the sole source of nitrogen. Mutants of S. cerevisiae with defective proline transport were isolated by selecting for resistance to either of the toxic proline analogs L-azetidine-2-carboxylate or 3,4-dehydro-DL-proline. Strains carrying the put4 mutation are defective in the high-affinity proline transport system. These mutants could still grow when given high concentrations of proline, due to the operation of low-affinity systems whose existence as confirmed by kinetic studies. Both systems were repressed by ammonium ions, and either was induce by proline. Low-affinity transport was inhibited by histidine, so put4 mutants were unable to grow on a medium containing high concentrations of proline to which histidine has been added.
摘要
酿酒酵母能够将脯氨酸作为唯一的氮源加以利用。通过筛选对有毒的脯氨酸类似物L-氮杂环丁烷-2-羧酸或3,4-脱氢-DL-脯氨酸之一具有抗性，分离得到了脯氨酸转运存在缺陷的酿酒酵母突变体。携带put4突变的菌株在高亲和力脯氨酸转运系统方面存在缺陷。由于低亲和力系统的运作（动力学研究证实了其存在），这些突变体在给予高浓度脯氨酸时仍能生长。这两种系统都受到铵离子的抑制，并且任何一种都可被脯氨酸诱导。低亲和力转运受到组氨酸的抑制，因此put4突变体无法在添加了组氨酸的高浓度脯氨酸培养基上生长。
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