Ortenblad N, Lunde P K, Levin K, Andersen J L, Pedersen P K
Institute of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense University, 5230 Odense M, Denmark.
Am J Physiol Regul Integr Comp Physiol. 2000 Jul;279(1):R152-60. doi: 10.1152/ajpregu.2000.279.1.R152.
To evaluate the effect of intermittent sprint training on sarcoplasmic reticulum (SR) function, nine young men performed a 5 wk high-intensity intermittent bicycle training, and six served as controls. SR function was evaluated from resting vastus lateralis muscle biopsies, before and after the training period. Intermittent sprint performance (ten 8-s all-out periods alternating with 32-s recovery) was enhanced 12% (P < 0.01) after training. The 5-wk sprint training induced a significantly higher (P < 0.05) peak rate of AgNO(3)-stimulated Ca(2+) release from 709 (range 560-877; before) to 774 (596-977) arbitrary units Ca(2+). g protein(-1). min(-1) (after). The relative SR density of functional ryanodine receptors (RyR) remained unchanged after training; there was, however, a 48% (P < 0.05) increase in total number of RyR. No significant differences in Ca(2+) uptake rate and Ca(2+)-ATPase capacity were observed following the training, despite that the relative density of Ca(2+)-ATPase isoforms SERCA1 and SERCA2 had increased 41% and 55%, respectively (P < 0.05). These data suggest that high-intensity training induces an enhanced peak SR Ca(2+) release, due to an enhanced total volume of SR, whereas SR Ca(2+) sequestration function is not altered.
为评估间歇性冲刺训练对肌浆网（SR）功能的影响，9名年轻男性进行了为期5周的高强度间歇性自行车训练，6人作为对照。在训练期前后，通过对股外侧肌静息肌肉活检来评估SR功能。训练后，间歇性冲刺表现（十个8秒全力冲刺期与32秒恢复期交替）提高了12%（P<0.01）。为期5周的冲刺训练使硝酸银刺激的Ca(2+)释放峰值速率显著升高（P<0.05），从709（范围560 - 877；训练前）升至774（596 - 977）任意单位Ca(2+).g蛋白(-1).min(-1)（训练后）。训练后功能性兰尼碱受体（RyR）的相对SR密度保持不变；然而，RyR总数增加了48%（P<0.05）。训练后未观察到Ca(2+)摄取速率和Ca(2+)-ATP酶活性的显著差异，尽管Ca(2+)-ATP酶亚型SERCA1和SERCA2的相对密度分别增加了41%和55%（P<0.05）。这些数据表明，高强度训练导致SR Ca(2+)释放峰值增强，这是由于SR总体积增加，而SR Ca(2+)螯合功能未改变。
