Section of Integrative Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark.
Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Elife. 2022 May 31;11:e69802. doi: 10.7554/eLife.69802.
Exercise is an effective strategy in the prevention and treatment of metabolic diseases. Alterations in the skeletal muscle proteome, including post-translational modifications, regulate its metabolic adaptations to exercise. Here, we examined the effect of high-intensity interval training (HIIT) on the proteome and acetylome of human skeletal muscle, revealing the response of 3168 proteins and 1263 lysine acetyl-sites on 464 acetylated proteins. We identified global protein adaptations to exercise training involved in metabolism, excitation-contraction coupling, and myofibrillar calcium sensitivity. Furthermore, HIIT increased the acetylation of mitochondrial proteins, particularly those of complex V. We also highlight the regulation of exercise-responsive histone acetyl-sites. These data demonstrate the plasticity of the skeletal muscle proteome and acetylome, providing insight into the regulation of contractile, metabolic and transcriptional processes within skeletal muscle. Herein, we provide a substantial hypothesis-generating resource to stimulate further mechanistic research investigating how exercise improves metabolic health.
运动是预防和治疗代谢性疾病的有效策略。骨骼肌蛋白质组的改变，包括翻译后修饰，调节其对运动的代谢适应。在这里，我们研究了高强度间歇训练（HIIT）对人类骨骼肌蛋白质组和乙酰组的影响，揭示了 3168 种蛋白质和 464 种乙酰化蛋白质上 1263 个赖氨酸乙酰化位点的反应。我们确定了与代谢、兴奋-收缩偶联和肌球蛋白钙敏感性相关的运动训练的全局蛋白质适应。此外，HIIT 增加了线粒体蛋白，特别是复合物 V 的乙酰化。我们还强调了运动反应性组蛋白乙酰化位点的调节。这些数据表明骨骼肌蛋白质组和乙酰组具有可塑性，为骨骼肌中收缩、代谢和转录过程的调节提供了深入了解。在此，我们提供了一个重要的假设生成资源，以激发进一步的机制研究，探讨运动如何改善代谢健康。
