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噬菌体φX174的感染过程。三十七。φX174感染细胞中的RNA代谢。

Process of infection with bacteriophage phiX174. XXXVII. RNA metabolism in phiX174-infected cells.

作者信息

Clements J B, Sinsheimer R L

出版信息

J Virol. 1975 Jan;15(1):151-60. doi: 10.1128/JVI.15.1.151-160.1975.

DOI:10.1128/JVI.15.1.151-160.1975
PMID:1089800
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC354429/
Abstract

The RNA produced in vivo from bacteriophage phiX174 DNA has been analyzed by polyacrylamide-agarose gel electrophoresis and sedimentation in dimethyl sulfoxide gradients, and the results of Hayashi and Hayashi (1970) have been confirmed and extended. An efficient procedure for recovery of RNA from gels, followed by a hybridization assay, has indicated the presence in infected cells of 18 distinct RNA species with sizes up to and greater than the unit (viral) length. The sizes of phiX mRNA's were similar irrespective of whether material was analyzed on gels or in dimethyl sulfoxide gradients. When virus-induced RNA was detected by a double-label method, seven additional low-molecular weight species were observed on gels and the resolution of dimethyl sulfoxide gradients was enhanced. The present results lend support to aspects of the model of Hayashi and Hayashi (1970) for the generation of these discrete mRNA species; an alternative model is also discussed.

摘要

利用聚丙烯酰胺 - 琼脂糖凝胶电泳和在二甲基亚砜梯度中的沉降分析了噬菌体φX174 DNA在体内产生的RNA，证实并扩展了Hayashi和Hayashi（1970）的结果。一种从凝胶中回收RNA的有效方法，随后进行杂交分析，表明在受感染细胞中存在18种不同的RNA种类，其大小达到并大于单位（病毒）长度。无论在凝胶上还是在二甲基亚砜梯度中分析材料，φX mRNA的大小都是相似的。当通过双标记法检测病毒诱导的RNA时，在凝胶上观察到另外七种低分子量种类，并且二甲基亚砜梯度的分辨率提高了。目前的结果支持了Hayashi和Hayashi（1970）关于这些离散mRNA种类产生的模型的某些方面；还讨论了另一种模型。

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