Rankinen T, Gagnon J, Pérusse L, Chagnon Y C, Rice T, Leon A S, Skinner J S, Wilmore J H, Rao D C, Bouchard C
Pennington Biomedical Research Center, Human Genomics Laboratory, Baton Rouge, Louisiana 70808, USA.
Am J Physiol Heart Circ Physiol. 2000 Jul;279(1):H368-74. doi: 10.1152/ajpheart.2000.279.1.H368.
We investigated the association between angiotensinogen (AGT) and angiotensin-converting enzyme (ACE) gene polymorphisms and exercise training responses of resting and exercise blood pressure (BP). BP at rest and during submaximal (50 watts) and maximal exercise tests was measured before and after 20 wk of endurance training in 476 sedentary normotensive Caucasian subjects from 99 families. AGT M235T and ACE insertion/deletion polymorphisms were typed with PCR-based methods. Men carrying the AGT MM and MT genotypes showed 3. 7 +/- 0.6 and 3.2 +/- 0.5 (SE) mmHg reductions, respectively, in diastolic BP at 50 watts (DBP(50)), whereas, in the TT homozygotes, the decrease was 0.4 +/- 1.0 mmHg (P = 0.016 for trend, adjusted for age, body mass index, and baseline DBP(50)). Men with the ACE DD genotype showed a slightly greater decrease in DBP(50) (4.4 +/- 0.6 mmHg) than the II and ID genotypes (2.8 +/- 0.7 and 2.4 +/- 0.5 mmHg, respectively, P = 0.050). Furthermore, a significant (P = 0.022) interaction effect between the AGT and ACE genes was noted for DBP(50); the AGT TT homozygotes carrying the ACE D allele showed no response to training. Men with the AGT TT genotype had greater (P = 0.007) diastolic BP (DBP) response to acute maximal exercise at baseline. However, the difference disappeared after the training period. No associations were found in women. These data suggest that, in men, the genetic variation in the AGT locus modifies the responsiveness of submaximal exercise DBP to endurance training, and interactions between the AGT and ACE loci can alter this response.
我们研究了血管紧张素原（AGT）和血管紧张素转换酶（ACE）基因多态性与静息血压和运动血压（BP）的运动训练反应之间的关联。在来自99个家庭的476名久坐不动的血压正常的白种人受试者中，在进行20周耐力训练前后，测量了静息状态下以及次最大强度（50瓦）和最大运动测试期间的血压。采用基于聚合酶链反应（PCR）的方法对AGT M235T和ACE插入/缺失多态性进行分型。携带AGT MM和MT基因型的男性在50瓦功率时舒张压（DBP(50)）分别降低了3.7±0.6和3.2±0.5（标准误）mmHg，而在TT纯合子中，降低幅度为0.4±1.0 mmHg（趋势P = 0.016，经年龄、体重指数和基线DBP(50)校正）。具有ACE DD基因型的男性在DBP(50)上的降低幅度（4.4±0.6 mmHg）略大于II和ID基因型（分别为2.8±0.7和2.4±0.5 mmHg，P = 0.050）。此外，对于DBP(50)，AGT和ACE基因之间存在显著的（P = 0.022）交互作用；携带ACE D等位基因的AGT TT纯合子对训练无反应。具有AGT TT基因型的男性在基线时对急性最大运动的舒张压（DBP）反应更大（P = 0.007）。然而，训练期后这种差异消失。在女性中未发现关联。这些数据表明，在男性中，AGT基因座的遗传变异改变了次最大运动DBP对耐力训练的反应性，并且AGT和ACE基因座之间的相互作用可以改变这种反应。
