Arai T, Lundborg G, Dahlin L B
Department of Orthopaedic Surgery, School of Medicine, Keio University, Tokyo, Japan.
Scand J Plast Reconstr Surg Hand Surg. 2000 Jun;34(2):101-8. doi: 10.1080/02844310050159936.
A long defect (15 mm) in rat sciatic nerve was repaired with a bioartificial nerve graft composed of a silicone tube and seven synthetic filaments of five types (polyamide, catgut, polydioxanone, and two types of polyglactin, normal and quickly-absorbed) inserted longitudinally into the tube. In all cases in which filaments were used a regenerating bridge was obtained in the tube after three months in contrast to empty silicone tubes, in which no structure was observed. There was a 6%-46% recovery of isometric muscle contractility of the anterior tibial and gastrocnemius muscles with positive pinch reflex test in most cases. Myelinated axons were seen in the regenerating tissue between the filaments but not directly in contact with them, and there were varying numbers of macrophages close to the filaments. Silicone tubes with filaments, regardless of type of filament, induced nerve tissue to regenerate and resulted in functional recovery through a 15 mm nerve gap not achieved with empty tubes. Nerve promoting factors may be applied to the filaments and the model is a valuable tool for further development of artificial nerve grafts.
用一种生物人工神经移植物修复大鼠坐骨神经的长缺损（15毫米），该移植物由硅胶管和纵向插入管内的七种合成细丝组成，细丝有五种类型（聚酰胺、肠线、聚二氧六环酮以及两种聚乙交酯，普通型和快速吸收型）。在所有使用细丝的情况下，三个月后在管内获得了再生桥，而空硅胶管内未观察到任何结构。大多数情况下，胫前肌和腓肠肌的等长肌肉收缩力恢复了6% - 46%，捏压反射试验呈阳性。在细丝之间的再生组织中可见有髓轴突，但它们并不直接与细丝接触，并且在细丝附近有数量不等的巨噬细胞。带有细丝的硅胶管，无论细丝类型如何，均可诱导神经组织再生，并通过15毫米的神经间隙实现功能恢复，而空管则无法做到这一点。神经促进因子可应用于细丝，该模型是进一步开发人工神经移植物的宝贵工具。
