Terada N, Bjursten L M, Dohi D, Lundborg G
Department of Orthopaedic Surgery, School of Medicine, Keio University, Tokyo, Japan.
Scand J Plast Reconstr Surg Hand Surg. 1997 Mar;31(1):1-6. doi: 10.3109/02844319709010498.
Gaps 10 mm wide in the sciatic nerves of 64 rats were bridged by bioartificial nerve grafts consisting of a silicone tube containing seven longitudinally placed filaments made of non-absorbable, (polyamide [Ethilon]) or absorbable, material (polydioxanone [PDS], polyglactin [Vicryl], and catgut). The purpose was to study the organisation of axonal growth inside the tube along such filaments. After two and four weeks histological techniques were used to study the contents of the tube and at four weeks immunohistological techniques were used to confirm the presence of axons distal to the tube. In all experimental groups axons had traversed the tube and reached the distal segment after four weeks. Inside the tube axons were organised in multiple minifascicles in all groups, but there were no axons growing in direct contact with the filaments. We conclude that resorbable filaments placed inside a silicone tube do not disturb axonal growth across the tube.
用生物人工神经移植物桥接64只大鼠坐骨神经上10毫米宽的间隙，该移植物由硅胶管组成，管内有七根纵向放置的细丝，细丝由不可吸收材料（聚酰胺[Ethilon]）或可吸收材料（聚二氧六环酮[PDS]、聚乙交酯[Vicryl]和肠线）制成。目的是研究管内轴突沿此类细丝生长的组织情况。两周和四周后，使用组织学技术研究管内的内容物，四周时使用免疫组织学技术确认管远端轴突的存在。在所有实验组中，四周后轴突都穿过了管子并到达了远端节段。在管内，所有组的轴突都组织成多个小束，但没有轴突与细丝直接接触生长。我们得出结论，置于硅胶管内的可吸收细丝不会干扰轴突穿过管子的生长。
