Lundborg G, Kanje M
Department of Hand Surgery, Malmö University Hospital, Sweden.
Scand J Plast Reconstr Surg Hand Surg. 1996 Jun;30(2):105-10. doi: 10.3109/02844319609056391.
A prototype for a bioartificial nerve graft has been developed. To set a baseline for a series of experimental studies we present a basic model in which multiple polyamide (nylon) filaments inside silicone tubes constitute an intrinsic and extrinsic framework, respectively, for regenerating axons. The prototype was used to bridge a 10 mm gap in rat sciatic nerve (n = 8). Four weeks postoperatively multiple axons organised into minifascicles were observed in all cases between the synthetic filaments and in the space between the filaments and the silicone tube. Pinching of the nerve distal to the prototype caused a response in 50% of the cases indicating the presence of regenerating sensory fibres compared with no response when no conduit had been used to bridge the gap. When an empty tube was used, pinching of the distal segment caused a response in all cases. The finding that regeneration is supported by the filament filled tubes forms a basis for further development of the bioartificial nerve graft concept with special reference to its potential for bridging extended gaps in nerve continuity.
一种生物人工神经移植物的原型已经研制出来。为了为一系列实验研究设定一个基线，我们提出了一个基本模型，其中硅管内的多根聚酰胺（尼龙）细丝分别构成了用于轴突再生的内在和外在框架。该原型被用于桥接大鼠坐骨神经10毫米的间隙（n = 8）。术后四周，在所有病例中，均可观察到合成细丝之间以及细丝与硅管之间的间隙中有多个轴突组织成小束。与未使用导管桥接间隙时无反应相比，在原型远端挤压神经，50%的病例出现反应，表明存在再生的感觉纤维。当使用空管时，挤压远端节段在所有病例中均引起反应。细丝填充管支持再生这一发现为生物人工神经移植物概念的进一步发展奠定了基础，特别是考虑到其在桥接神经连续性中长间隙方面的潜力。
