Schulz K H, Hausen B M, Wallhöfer L, Schmidt-Löffler P
Arch Dermatol Forsch. 1975;251(3):235-44.
Experimental studies on allergic contact dermatitis due to Chrysanthemum indicum 1. (Chrysanthemum x-morifolium) (chrysanthemum of the florists) have indicated the following results: 1. In 5 patients with allergic contact dermatitis from chrysanthemum oil of turpentine and its sensitizing compounds gave no patch test responses. No relationship between contact allergy due to chrysanthemus and to turpentine oil could be determined. 2. The pyrethrins, constituents with insecticidal activity, derived from certain Chrysanthemum species and often suspected as the causative agents, play no role in chrysanthemum allergy. 3. Tests on sensitized guinea pigs (pirl bright white strain) with flowers of chrysanthemum as well as with the two sesquiterpene lactones parthenolide and alantolactone, derived from different Composite species, gave positive patch test reactions. The results showed that parthenolide produced stronger reactions than alantolactone. 4. By thin layer chromatography neither parthenolide, nor alantolactone or pyrethrosin could be detected in extracts of chrysanthemum flowers of the florists. But the investigations indicated that several other terpenic compounds are present, which gave positive color reactions to certain lactone reagents. Five of them showed strong positive patch test reactions in our patients as well as in sensitized guinea pigs. Further studies are required to identify these compounds.
野菊花（菊花×菊）（花店售卖的菊花）引起过敏性接触性皮炎的实验研究表明了以下结果：1. 5例对松节油菊花油及其致敏化合物产生过敏性接触性皮炎的患者，斑贴试验无反应。无法确定菊花和松节油引起的接触性过敏之间的关系。2. 某些菊花品种中具有杀虫活性的成分除虫菊酯，常被怀疑是致病因素，但在菊花过敏中不起作用。3. 用菊花花朵以及从不同菊科植物中提取的两种倍半萜内酯小白菊内酯和土木香内酯对致敏豚鼠（珍珠亮白品系）进行试验，斑贴试验呈阳性反应。结果表明，小白菊内酯产生的反应比土木香内酯更强。4. 通过薄层色谱法，在花店售卖的菊花花朵提取物中未检测到小白菊内酯、土木香内酯或除虫菊酯。但研究表明存在其他几种萜类化合物，它们对某些内酯试剂呈阳性颜色反应。其中五种在我们的患者以及致敏豚鼠中表现出强烈的阳性斑贴试验反应。需要进一步研究来鉴定这些化合物。
