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使用叶绿素和溴化乙锭荧光通过流动式微荧光计分离藻类混合物和细菌混合物。

Separation of algal mixtures and bacterial mixtures with flow-microfluorometer using chlorophyll and ethidium bromide fluorescence.

作者信息

Paau A S, Cowles J R, Oro J, Bartel A, Hungerford E

出版信息

Arch Microbiol. 1979 Mar 12;120(3):271-3. doi: 10.1007/BF00423075.

DOI:10.1007/BF00423075
PMID:109060
Abstract

The applicability of flow-microfluorometer to separate microbial cells was demonstrated with algal and bacterial cells. Algal mixtures were sorted according to the natural chlorophyll fluorescence and the bacterial mixtures were sorted according to the fluorescence of ethidium bromide-stained nucleic acid.

摘要

流式微量荧光计对藻类和细菌细胞进行微生物细胞分离的适用性得到了证实。藻类混合物根据天然叶绿素荧光进行分选，细菌混合物根据溴化乙锭染色核酸的荧光进行分选。

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引用本文的文献

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Flow cytometry of spinach chloroplasts : determination of intactness and lectin-binding properties of the envelope and the thylakoid membranes.菠菜叶绿体的流式细胞术分析：质膜和类囊体膜的完整性和凝集素结合特性的测定。
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本文引用的文献

1
A new multiparameter separator for microscopic particles and biological cells.一种用于微观粒子和生物细胞的新型多参数分离器。
Rev Sci Instrum. 1973 Sep;44(9):1301-10. doi: 10.1063/1.1686375.
2
Chromosome measurement and sorting by flow systems.通过流动系统进行染色体测量和分选。
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1231-4. doi: 10.1073/pnas.72.4.1231.
3
Quantitative single cell analysis and sorting.定量单细胞分析与分选
Science. 1977 Oct 14;198(4313):149-57. doi: 10.1126/science.905822.
4
Comparison of nucleic acid content in populations of free-living and symbiotic Rhizobium meliloti by flow microfluorometry.通过流动微荧光法比较自由生活型和共生型苜蓿根瘤菌群体中的核酸含量。
J Bacteriol. 1977 Feb;129(2):1156-8. doi: 10.1128/jb.129.2.1156-1158.1977.
5
Characterization of bacterial growth by means of flow microfluorometry.
Science. 1977 Dec 16;198(4322):1175-6. doi: 10.1126/science.412254.
6
Flow-microfluorometric analysis of Escherichia coli, Rhizobium meliloti, and Rhizobium japonicum at different stages of the growth cycle.
Can J Microbiol. 1977 Sep;23(9):1165-9. doi: 10.1139/m77-175.