通过流动微荧光法比较自由生活型和共生型苜蓿根瘤菌群体中的核酸含量。
Comparison of nucleic acid content in populations of free-living and symbiotic Rhizobium meliloti by flow microfluorometry.
作者信息
Paau A S, Lee D, Cowles J R
出版信息
J Bacteriol. 1977 Feb;129(2):1156-8. doi: 10.1128/jb.129.2.1156-1158.1977.
Abstract
Populations of symbiotic Rhizobium meliloti extracted from alfalfa nodules were shown by flow microfluorometry to contain a significant number of bacteroids with higher nucleic acid content than the free-living rhizobia. Bacteroids with lower nucleic acid content than the free-living bacteria were not detected in significant quantities in these populations. These results indicate that the incapability of bacteroids to reestablish growth in nutrient media may not be caused by a decrease in nucleic acid content of the symbiotic rhizobia.
摘要
通过流动微荧光测定法显示，从苜蓿根瘤中提取的共生苜蓿中华根瘤菌群体含有大量类菌体，其核酸含量高于自由生活的根瘤菌。在这些群体中未大量检测到核酸含量低于自由生活细菌的类菌体。这些结果表明，类菌体在营养培养基中无法重新建立生长可能不是由共生根瘤菌核酸含量的降低引起的。
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