Terawaki Y, Kishi H, Nakaya R
J Bacteriol. 1975 Mar;121(3):857-62. doi: 10.1128/jb.121.3.857-862.1975.
An R plasmid Rts1 was integrated into the gal region of the chromosome of Escherichia coli XA-7012 (galE) strain by the directed transposition technique. The integration of the Rts1 genome was confirmed mainly by conjugation studies and also by transduction experiments using phage P1. As a result, it was found that the integrated genome contained genes responsible for kanamycin resistance, conjugal transferability, and for autonomous replication. As reported previously, Rts1 is temperature sensitive in replication and inhibits the growth of the host at nonpermissive temperature. However, although a plasmid derived from the integrated Rts1 genome still demonstrates temperature sensitivity upon transfer and high level of kanamycin resistance, this plasmid no longer displays temperature sensitivity in replication and the inhibitory effect on the host. These results indicate that the temperature sensitivity of replication of Rts1 and its inhibitory effect on the host cell are due to the presence of a gene or gene cluster on the Rts1 genome and that the gene(s) is clearly discriminated from the one responsible for the temperature sensitivity of transfer.
通过定向转座技术，将R质粒Rts1整合到大肠杆菌XA - 7012（galE）菌株染色体的gal区域。Rts1基因组的整合主要通过接合研究以及使用噬菌体P1的转导实验得以证实。结果发现，整合后的基因组包含负责卡那霉素抗性、接合转移性和自主复制的基因。如先前报道，Rts1在复制方面对温度敏感，并且在非允许温度下会抑制宿主生长。然而，尽管源自整合的Rts1基因组的质粒在转移时仍表现出温度敏感性以及高水平的卡那霉素抗性，但该质粒在复制时不再表现出温度敏感性，对宿主也不再有抑制作用。这些结果表明，Rts1复制的温度敏感性及其对宿主细胞的抑制作用是由于Rts1基因组上存在一个基因或基因簇，并且该基因（或这些基因）与负责转移温度敏感性的基因明显不同。
