Nakazawa T
J Bacteriol. 1978 Feb;133(2):527-35. doi: 10.1128/jb.133.2.527-535.1978.
The TOL plasmid originally isolated in Pseudomonas putida (arvilla) mt-2 was transmissible to strains of the fluorescens group of Pseudomonas, i.e., P. putida, P. fluorescens, and P. aeruginosa, except for a strain of P. aeruginosa, strain PAO. The same strain, however, could accept the plasmid when its restriction and modification abilities were lost by mutations or by growing at high temperature. In addition, the transmissibility of the TOL plasmid from strain PAO to P. putida was low when the plasmid was modified by the donor. By using P. aeruginosa PAO carrying the TOL plasmid, the stability and genetic expression of the plasmid as well as its effect on the host cell growth were examined. Thus the self-maintenance of the plasmid was found to be thermosensitive. Furthermore, the TOL plasmid inhibited the growth of strain PAO at high temperature, accompanied by the formation of some filamentous cells. These thermosensitive properties of the TOL plasmid were host dependent and not exhibited in another strain of P. aeruginosa.
最初从恶臭假单胞菌（阿维拉）mt-2中分离出的TOL质粒可转移至荧光假单胞菌群的菌株，即恶臭假单胞菌、荧光假单胞菌和铜绿假单胞菌，但铜绿假单胞菌的PAO菌株除外。然而，当同一菌株因突变或在高温下生长而丧失其限制和修饰能力时，它可以接受该质粒。此外，当供体对TOL质粒进行修饰时，该质粒从PAO菌株转移至恶臭假单胞菌的能力较低。通过使用携带TOL质粒的铜绿假单胞菌PAO，研究了该质粒的稳定性、遗传表达及其对宿主细胞生长的影响。结果发现该质粒的自我维持具有热敏感性。此外，TOL质粒在高温下抑制PAO菌株的生长，并伴有一些丝状细胞的形成。TOL质粒的这些热敏感特性依赖于宿主，在另一株铜绿假单胞菌中未表现出来。
