Goto N, Yoshida Y, Terawaki Y, Nakaya R, Suzuki K
J Bacteriol. 1970 Mar;101(3):856-9. doi: 10.1128/jb.101.3.856-859.1970.
The buoyant density of deoxyribonucleic acid (DNA) from the temperature-sensitive R factor, Rts1, was determined by CsCl density-gradient centrifugation. Rts1 was found to consist of a single species of DNA of density 1.705 g/cm(3), which corresponds to a base composition of 45% guanine plus cytosine. This value is distinct from the densities previously reported for other R factors, suggesting that Rts1 represents a new molecular class of R factors.
通过氯化铯密度梯度离心法测定了温度敏感型R因子Rts1的脱氧核糖核酸(DNA)的浮力密度。发现Rts1由单一类型的DNA组成,其密度为1.705克/立方厘米,这对应于45%的鸟嘌呤加胞嘧啶的碱基组成。该值与先前报道的其他R因子的密度不同,表明Rts1代表了一种新的R因子分子类别。