Marca M C, Loste A
J Small Anim Pract. 2000 May;41(5):189-92. doi: 10.1111/j.1748-5827.2000.tb03192.x.
A method for assaying canine glycosylated haemoglobin was evaluated. The method is a turbidimetric inhibition immunoassay and the final reaction is bichromatically measured using a multichannel automatic analyser. Within-run coefficients of variation (2.07 to 4.46 per cent) were permissible, but between-run coefficients of variation (2.10 to 8.25 per cent) were slightly more elevated. The detection limit of this assay is 0.052 per cent. A sample dilution of 10 microliters of sample and 500 microliters of haemolysing reagent is recommended for routine analysis of canine blood samples. A normal reference interval of 1.39 +/- 0.70 per cent was obtained from the glycosylated haemoglobin analysis in 82 healthy dogs and no statistically significant differences in relation to age or gender were observed. Some changes in glycosylated haemoglobin concentrations were noted throughout the ovarian cycle, although differences between dogs were evident. Since this assay specifically measures the glycosylated haemoglobin content in canine blood samples, it could be very useful for monitoring diabetic dogs.
对一种检测犬糖化血红蛋白的方法进行了评估。该方法为比浊抑制免疫测定法，最终反应使用多通道自动分析仪进行双色测量。批内变异系数（2.07%至4.46%）是可接受的，但批间变异系数（2.10%至8.25%）略高。该检测方法的检测限为0.052%。建议将10微升样品和500微升溶血试剂进行样品稀释，用于犬血样的常规分析。从82只健康犬的糖化血红蛋白分析中获得的正常参考区间为1.39±0.70%，未观察到与年龄或性别相关的统计学显著差异。在整个卵巢周期中，糖化血红蛋白浓度有一些变化，尽管犬之间的差异很明显。由于该检测方法专门测量犬血样中的糖化血红蛋白含量，因此对监测糖尿病犬可能非常有用。
