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一种经噬菌体T5修饰的大肠杆菌RNA聚合酶的纯化及特性

Purification and properties of a bacteriophage T5-modified form of Escherichia coli RNA polymerase.

作者信息

Szabo C, Moyer R W

出版信息

J Virol. 1975 Apr;15(4):1042-6. doi: 10.1128/JVI.15.4.1042-1046.1975.

DOI:10.1128/JVI.15.4.1042-1046.1975
PMID:1090747
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC354552/
Abstract

A modified form of Escherichia coli RNA polymerase that contains one of the three T5-specific polypeptides known to interact with the host enzyme was purified from bacteriophage T5-infected cells. The properties of this T5-modified enzyme appeared identical to those of the RNA polymerase derived from uninfected non-colicinogenic cells and to a fully active enzyme isolated from T5-infected ColIb+ cells after the limited in vivo transcription of T5 genes allowed by the plasmid had ceased.

摘要

一种经过修饰的大肠杆菌RNA聚合酶从被噬菌体T5感染的细胞中纯化出来，该酶含有已知与宿主酶相互作用的三种T5特异性多肽中的一种。这种T5修饰酶的特性似乎与来自未感染的非产大肠杆菌素细胞的RNA聚合酶相同，也与在质粒允许的T5基因进行有限的体内转录停止后，从T5感染的ColIb+细胞中分离出的完全活性酶相同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4f7/354552/39d4d7edc427/jvirol00232-0380-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4f7/354552/39d4d7edc427/jvirol00232-0380-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4f7/354552/39d4d7edc427/jvirol00232-0380-a.jpg

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