Glenn J, Duckworth D H
J Virol. 1979 May;30(2):421-30. doi: 10.1128/JVI.30.2.421-430.1979.
T5 bacteriophage cannot replicate in Escherichia coli containing the colicinogenic factor ColIb. We show that active transport of proline and glutamine begins to decline at about 10 min after infection, the same time at which macromolecular synthesis stops during abortive infection. Uptake of alpha-methylglucoside is stimulated, however, and this change is evident even by 5 min after infection. These changes in membrane function do not occur during infections that are productive because of mutations on the plasmid or phage. The results suggest that the abortive infection is caused by membrane depolarization.
T5噬菌体不能在含有产大肠杆菌素因子ColIb的大肠杆菌中复制。我们发现，脯氨酸和谷氨酰胺的主动转运在感染后约10分钟开始下降，这与流产感染期间大分子合成停止的时间相同。然而，α-甲基葡萄糖苷的摄取受到刺激，甚至在感染后5分钟这种变化就很明显。在由于质粒或噬菌体上的突变而产生的有效感染过程中，膜功能不会发生这些变化。结果表明，流产感染是由膜去极化引起的。
