Kos K A, Osborne B A, Goldsby R A
J Virol. 1975 Apr;15(4):913-7. doi: 10.1128/JVI.15.4.913-917.1975.
A comparative study of the growth of Sindbis (SIN) virus, a group A arbovirus (togavirus), and Japanese encephalitis (JE) virus, a representative group B arbovirus (togavirus), was conducted in enucleate and nucleate cells. Immunofluorescent tests and yield measurements demonstrated that chicken embryo cells which had been enucleated and subsequently infected with SIN virus produced virus-specific antigens and infectious virus. By contrast, JE failed to replicate or produce virus-specific antigen in cells which had been enucleated before or even 2 h post infection. Studies of the effect of enulceation at various times after infection demonstrated that a nucleus must be present at least 2 and possibly as long as 4 h after infection to produce either JE-specific antigen or infectious JE virus. These studies demonstrate that the replication of SIN, a group A arbovirus (togavirus), which has no nuclear requirement, contrasts sharply with that of a group B arbovirus (togavirus), JE, which may have an initial dependence on a nucleus-associated process.
对A组虫媒病毒（披膜病毒科）辛德毕斯（SIN）病毒和B组虫媒病毒（披膜病毒科）的代表性病毒日本脑炎（JE）病毒在去核细胞和有核细胞中的生长情况进行了比较研究。免疫荧光试验和产量测量表明，去核后再感染SIN病毒的鸡胚细胞产生了病毒特异性抗原和传染性病毒。相比之下，JE病毒在感染前甚至感染后2小时去核的细胞中无法复制或产生病毒特异性抗原。对感染后不同时间去核效果的研究表明，感染后至少2小时且可能长达4小时必须存在细胞核才能产生JE特异性抗原或传染性JE病毒。这些研究表明，对细胞核无需求的A组虫媒病毒（披膜病毒科）SIN病毒的复制，与可能最初依赖细胞核相关过程的B组虫媒病毒（披膜病毒科）JE病毒的复制形成鲜明对比。
