Gupta A K, Gore M M, Lad V J, Ghosh S N
National Institute of Virology, Pune, India.
Acta Virol. 1991 May;35(3):282-6.
Acetone-fixed porcine stable kidney (PS) cells infected with Japanese encephalitis (JE) virus were stained in indirect fluorescent antibody (FA) assay with anti-JE virus monoclonal (MoAb) and polyclonal (immune PF) antibodies. First positive immunofluorescence (IF) occurred in the cytoplasm with MoAb Hs-1 (anti-envelope, JE-specific) and immune PF after 7 hr post-infection (p. i.); it became prominent by 15 hr to 48 hr (maximum) when cells reacted strongly also with MoAb Hx-3 (flavivirus crossreactive epitope). In addition, 15 to 20% of the infected cells, which revealed positive cytoplasmic IF, showed intranuclear IF with Hs-1, Hx-3, and immune PF by 20 to 24 hr p.i. By 48 hr, the intranuclear IF was not observed or became diminished. These observations indicate that the JE virus specific epitope Hs-1 appeared first followed by the flavivirus cross-reactive epitope Hx-3. Nuclei of the infected cells seem to play some role in the replication of JE virus.
用抗日本脑炎（JE）病毒单克隆抗体（MoAb）和多克隆抗体（免疫PF），在间接荧光抗体（FA）试验中对感染了JE病毒的丙酮固定猪稳定肾（PS）细胞进行染色。感染后7小时（p.i.），用MoAb Hs-1（抗包膜，JE特异性）和免疫PF在细胞质中首次出现阳性免疫荧光（IF）；当细胞在15小时至48小时（最大值）时也与MoAb Hx-3（黄病毒交叉反应表位）强烈反应时，阳性免疫荧光变得明显。此外，15%至20%显示细胞质IF阳性的感染细胞，在感染后20至24小时，用Hs-1、Hx-3和免疫PF显示核内IF。到48小时，未观察到核内IF或其减弱。这些观察结果表明，JE病毒特异性表位Hs-1首先出现，随后是黄病毒交叉反应表位Hx-3。感染细胞的细胞核似乎在JE病毒的复制中起一定作用。
