• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Mutational analyses of dinucleotide and tetranucleotide microsatellites in Escherichia coli: influence of sequence on expansion mutagenesis.大肠杆菌中二核苷酸和四核苷酸微卫星的突变分析：序列对扩增诱变的影响。
Nucleic Acids Res. 2000 Jul 15;28(14):2831-8. doi: 10.1093/nar/28.14.2831.
2
Somatic mutation rates and specificities at TC/AG and GT/CA microsatellite sequences in nontumorigenic human lymphoblastoid cells.非致瘤性人淋巴母细胞中TC/AG和GT/CA微卫星序列的体细胞突变率及特异性
Cancer Res. 2000 Mar 15;60(6):1698-703.
3
Mutation rate and specificity analysis of tetranucleotide microsatellite DNA alleles in somatic human cells.人体体细胞中四核苷酸微卫星DNA等位基因的突变率和特异性分析。
Mol Carcinog. 2002 Jul;34(3):140-50. doi: 10.1002/mc.10058.
4
Misalignment-mediated DNA polymerase beta mutations: comparison of microsatellite and frame-shift error rates using a forward mutation assay.错配介导的DNA聚合酶β突变：使用正向突变试验比较微卫星和移码错误率
Biochemistry. 2002 Aug 20;41(33):10490-8. doi: 10.1021/bi025918c.
5
Escherichia coli DNA polymerase IV contributes to spontaneous mutagenesis at coding sequences but not microsatellite alleles.大肠杆菌DNA聚合酶IV对编码序列的自发诱变有作用，但对微卫星等位基因没有作用。
Mutat Res. 2007 Jun 1;619(1-2):93-103. doi: 10.1016/j.mrfmmm.2007.02.007. Epub 2007 Mar 2.
6
Mutations in polI but not mutSLH destabilize Haemophilus influenzae tetranucleotide repeats.聚合酶I的突变而非mutSLH的突变会使流感嗜血杆菌的四核苷酸重复序列不稳定。
EMBO J. 2002 Mar 15;21(6):1465-76. doi: 10.1093/emboj/21.6.1465.
7
Human postmeiotic segregation 2 exhibits biased repair at tetranucleotide microsatellite sequences.人类减数分裂后分离2在四核苷酸微卫星序列处表现出偏向性修复。
Cancer Res. 2009 Feb 1;69(3):1143-9. doi: 10.1158/0008-5472.CAN-08-3499. Epub 2009 Jan 20.
8
Relative rates of insertion and deletion mutations in dinucleotide repeats of various lengths in mismatch repair proficient mouse and mismatch repair deficient human cells.错配修复功能正常的小鼠细胞和错配修复功能缺陷的人类细胞中不同长度二核苷酸重复序列的插入和缺失突变相对速率。
Mutat Res. 2002 Feb 20;499(2):213-25. doi: 10.1016/s0027-5107(01)00282-2.
9
Effects of oxidative and alkylating damage on microsatellite instability in nontumorigenic human cells.氧化和烷基化损伤对非致瘤性人类细胞微卫星不稳定性的影响。
Mutat Res. 2004 Feb 26;546(1-2):29-38. doi: 10.1016/j.mrfmmm.2003.10.001.
10
Sequence dependent instability of mononucleotide microsatellites in cultured mismatch repair proficient and deficient mammalian cells.培养的错配修复功能正常和缺陷的哺乳动物细胞中单核核苷酸微卫星序列依赖性不稳定性
Hum Mol Genet. 2002 Mar 15;11(6):707-13. doi: 10.1093/hmg/11.6.707.

引用本文的文献

1
Phasome analysis of pathogenic and commensal Neisseria species expands the known repertoire of phase variable genes, and highlights common adaptive strategies.致病性奈瑟菌和共生奈瑟菌的相变体分析扩展了已知的相变体基因库，并突出了常见的适应性策略。
PLoS One. 2018 May 15;13(5):e0196675. doi: 10.1371/journal.pone.0196675. eCollection 2018.
2
Microsatellite Length Scoring by Single Molecule Real Time Sequencing - Effects of Sequence Structure and PCR Regime.通过单分子实时测序进行微卫星长度评分——序列结构和PCR条件的影响
PLoS One. 2016 Jul 14;11(7):e0159232. doi: 10.1371/journal.pone.0159232. eCollection 2016.
3
Comparison of southern Chinese Han and Brazilian Caucasian mutation rates at autosomal short tandem repeat loci used in human forensic genetics.中国南方汉族和巴西白种人常染色体短串联重复序列位点突变率的比较，这些位点用于人类法医遗传学。
Int J Legal Med. 2014 Jan;128(1):1-9. doi: 10.1007/s00414-013-0847-2. Epub 2013 Apr 3.
4
A study on mutational dynamics of simple sequence repeats in relation to mismatch repair system in prokaryotic genomes.原核基因组中单核苷酸重复序列突变动态与错配修复系统的关系研究。
J Mol Evol. 2012 Apr;74(3-4):127-39. doi: 10.1007/s00239-012-9491-6. Epub 2012 Mar 14.
5
What is a microsatellite: a computational and experimental definition based upon repeat mutational behavior at A/T and GT/AC repeats.什么是微卫星：基于 A/T 和 GT/AC 重复的突变行为的计算和实验定义。
Genome Biol Evol. 2010;2:620-35. doi: 10.1093/gbe/evq046. Epub 2010 Jul 28.
6
The nucleotide composition of microsatellites impacts both replication fidelity and mismatch repair in human colorectal cells.微卫星的核苷酸组成影响人类结直肠细胞的复制保真度和错配修复。
Hum Mol Genet. 2010 Jul 1;19(13):2648-57. doi: 10.1093/hmg/ddq175. Epub 2010 Apr 26.
7
Both microsatellite length and sequence context determine frameshift mutation rates in defective DNA mismatch repair.微卫星长度和序列上下文决定有缺陷的 DNA 错配修复中的移码突变率。
Hum Mol Genet. 2010 Jul 1;19(13):2638-47. doi: 10.1093/hmg/ddq151. Epub 2010 Apr 23.
8
Distribution of polymorphic and non-polymorphic microsatellite repeats in Xenopus tropicalis.热带爪蟾中多态性和非多态性微卫星重复序列的分布
Bioinform Biol Insights. 2008 Feb 26;2:157-69. doi: 10.4137/bbi.s561.
9
Every microsatellite is different: Intrinsic DNA features dictate mutagenesis of common microsatellites present in the human genome.每个微卫星都是不同的：内在的DNA特征决定了人类基因组中常见微卫星的诱变作用。
Mol Carcinog. 2009 Apr;48(4):379-88. doi: 10.1002/mc.20499.
10
Human postmeiotic segregation 2 exhibits biased repair at tetranucleotide microsatellite sequences.人类减数分裂后分离2在四核苷酸微卫星序列处表现出偏向性修复。
Cancer Res. 2009 Feb 1;69(3):1143-9. doi: 10.1158/0008-5472.CAN-08-3499. Epub 2009 Jan 20.

本文引用的文献

1
Somatic mutation rates and specificities at TC/AG and GT/CA microsatellite sequences in nontumorigenic human lymphoblastoid cells.非致瘤性人淋巴母细胞中TC/AG和GT/CA微卫星序列的体细胞突变率及特异性
Cancer Res. 2000 Mar 15;60(6):1698-703.
2
Correspondence re: C.R. Boland et al., A National Cancer Institute workshop on microsatellite instability for cancer detection and familial predisposition: development of international criteria for the determination of microsatellite instability in colorectal cancer. Cancer Res., 58: 5248-5257, 1998.致信：C.R. 博兰等人，美国国立癌症研究所关于微卫星不稳定性在癌症检测和家族易感性方面的研讨会：结直肠癌微卫星不稳定性判定国际标准的制定。《癌症研究》，58卷：5248 - 5257页，1998年
Cancer Res. 1999 Jan 1;59(1):249-56.
3
A National Cancer Institute Workshop on Microsatellite Instability for cancer detection and familial predisposition: development of international criteria for the determination of microsatellite instability in colorectal cancer.美国国立癌症研究所微卫星不稳定性用于癌症检测和家族易感性研讨会：制定结直肠癌微卫星不稳定性测定的国际标准。
Cancer Res. 1998 Nov 15;58(22):5248-57.
4
Mutation rate of a microsatellite sequence in normal human fibroblasts.正常人成纤维细胞中微卫星序列的突变率。
Cancer Res. 1998 Sep 1;58(17):3946-9.
5
Equilibrium distributions of microsatellite repeat length resulting from a balance between slippage events and point mutations.由滑动事件和点突变之间的平衡所导致的微卫星重复长度的平衡分布。
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10774-8. doi: 10.1073/pnas.95.18.10774.
6
Yeast ARMs (DNA at-risk motifs) can reveal sources of genome instability.酵母ARMs（基因组不稳定风险基序）能够揭示基因组不稳定的来源。
Mutat Res. 1998 May 25;400(1-2):45-58. doi: 10.1016/s0027-5107(98)00047-5.
7
Trinucleotide repeat DNA structures: dynamic mutations from dynamic DNA.三核苷酸重复DNA结构：源于动态DNA的动态突变
Curr Opin Struct Biol. 1998 Jun;8(3):321-30. doi: 10.1016/s0959-440x(98)80065-1.
8
GAA instability in Friedreich's Ataxia shares a common, DNA-directed and intraallelic mechanism with other trinucleotide diseases.弗里德赖希共济失调中的GAA不稳定与其他三核苷酸疾病具有共同的、由DNA指导的等位基因内机制。
Mol Cell. 1998 Mar;1(4):583-93. doi: 10.1016/s1097-2765(00)80058-1.
9
Differences and similarities between various tandem repeat sequences: minisatellites and microsatellites.各种串联重复序列之间的差异与相似之处：小卫星和微卫星。
Biochimie. 1997 Oct;79(9-10):577-86. doi: 10.1016/s0300-9084(97)82006-8.
10
Hypermutability of homonucleotide runs in mismatch repair and DNA polymerase proofreading yeast mutants.错配修复和DNA聚合酶校对酵母突变体中同核苷酸序列的高突变性
Mol Cell Biol. 1997 May;17(5):2859-65. doi: 10.1128/MCB.17.5.2859.

大肠杆菌中二核苷酸和四核苷酸微卫星的突变分析：序列对扩增诱变的影响。

Mutational analyses of dinucleotide and tetranucleotide microsatellites in Escherichia coli: influence of sequence on expansion mutagenesis.

作者信息

Eckert K A, Yan G

机构信息

The Jake Gittlen Cancer Research Institute, The Pennsylvania State University College of Medicine, PO Box 850, Hershey, PA 17033, USA.

出版信息

Nucleic Acids Res. 2000 Jul 15;28(14):2831-8. doi: 10.1093/nar/28.14.2831.

DOI:10.1093/nar/28.14.2831
PMID:10908342
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC102660/
Abstract

Mutagenesis at GT/CA, TC/AG and TTCC/AAGG microsatellite sequences inserted in the herpes simplex virus thymidine kinase (HSV-tk) gene was analyzed in isogenic mutL(+) and mutL(-) Escherichia coli. In both strains, significantly more expansion than deletion mutations were observed at the TTCC/AAGG motif relative to either dinucleotide motif. As the HSV-tk coding sequence contains an endogenous G/C mononucleotide repeat and approximately 1000 bp of unique sequence, we were able to compare mutagenesis among various sequence motifs. We observed that the relative risk of mutation in E.COLI: is: TTCC/AAGG > GT/CA approximately TC/AG > unique approximately G/C. The mutation frequency varied 1400-fold in mutL(+) cells between the tetranucleotide motif and the mononucleotide motif, but only 50-fold in mutL(-) cells. The G/C sequence was destabilized the greatest and the tetranucleotide motif the least by loss of mismatch repair. These results demonstrate that the quantitative risk of mutation at various microsatellites greatly depends on the DNA sequence composition. We suggest alternative models for the production of expansion mutations during lagging strand replication of the TTCC/AAGG microsatellite.

摘要

在同基因的mutL(+)和mutL(-)大肠杆菌中，分析了插入单纯疱疹病毒胸苷激酶（HSV-tk）基因的GT/CA、TC/AG和TTCC/AAGG微卫星序列的诱变情况。在这两种菌株中，相对于任何一种二核苷酸基序，在TTCC/AAGG基序处观察到的扩增突变明显多于缺失突变。由于HSV-tk编码序列包含一个内源性的G/C单核苷酸重复序列和约1000 bp的独特序列，我们能够比较不同序列基序之间的诱变情况。我们观察到大肠杆菌中突变的相对风险为：TTCC/AAGG > GT/CA 约等于 TC/AG > 独特序列 约等于 G/C。在mutL(+)细胞中，四核苷酸基序和单核苷酸基序之间的突变频率相差1400倍，但在mutL(-)细胞中仅相差50倍。由于错配修复的丧失，G/C序列最不稳定，四核苷酸基序最稳定。这些结果表明，各种微卫星处突变的定量风险在很大程度上取决于DNA序列组成。我们提出了在TTCC/AAGG微卫星滞后链复制过程中产生扩增突变的替代模型。