Geuna S, Tos P, Battiston B, Guglielmone R, Giacobini-Robecchi M G
Dipartimento di Scienze Cliniche e Biologiche, Università di Torino, Ospedale San Luigi, Italy.
Anat Embryol (Berl). 2000 Jun;201(6):475-82. doi: 10.1007/s004290050334.
Clinical data have shown that a vein segment filled with fresh skeletal muscle can be considered a good autologous grafting conduit for the repair of peripheral nerve lesions. In this study, the long-term morphological organization of rat sciatic nerve fibers regenerated along a muscle-vein-combined graft conduit is further analysed by light and electron microscopy. Regenerated nerve fibers were organized into fascicles of various sizes that were clearly delimited by perineurial-like shells made by long and thin cytoplasmic processes of perineurial-like bipolar cells and by densely packed collagen fibrils. Grafted skeletal muscle fibers were still detectable among nerve fiber fascicles. However, in spite of the persistence of skeletal muscle along the graft, regenerated nerve fibers showed a good morphological pattern of regeneration, providing further evidence that the muscle-vein-combined grafting technique represents an effective surgical alternative to the classical fresh nerve autograft for the repair of peripheral nerve defects.
临床数据表明，一段充满新鲜骨骼肌的静脉段可被视为修复周围神经损伤的良好自体移植管道。在本研究中，通过光学显微镜和电子显微镜进一步分析了沿肌肉 - 静脉联合移植管道再生的大鼠坐骨神经纤维的长期形态组织。再生神经纤维被组织成各种大小的束，这些束由类神经束膜双极细胞的细长细胞质突起和紧密堆积的胶原纤维形成的类神经束膜壳清晰界定。在神经纤维束中仍可检测到移植的骨骼肌纤维。然而，尽管移植管道中骨骼肌持续存在，但再生神经纤维显示出良好的再生形态模式，这进一步证明肌肉 - 静脉联合移植技术是修复周围神经缺损的经典新鲜神经自体移植的有效手术替代方法。
