Shadduck R K, Waheed A, Pigoli G, Boegel F, Higgins L
Blood. 1979 Jun;53(6):1182-90.
Purified L-cell colony-stimulating factor (CSF) was coupled to cyanogen-bromide-activated Sepharose and used to selectively fractionate antibodies to this factor. With the use of a simplified two-step washing and elution technique, there was 50%--70% binding of the anti-CSF, with recovery of 60%--100% of the bound material. Both the native antiserum and purified anti-CSF fractions were inhibitory to murine granulocyte-macrophage colony formation. The purified antibodies contained only IgG and were reduced in protein concentration to 0.1% of the serum IgG values. These fractions should prove useful tools for the study of granulocyte and macrophage differentiation.
纯化的L细胞集落刺激因子（CSF）与溴化氰活化的琼脂糖偶联，用于选择性分离针对该因子的抗体。使用简化的两步洗涤和洗脱技术，抗CSF的结合率为50% - 70%，结合物质的回收率为60% - 100%。天然抗血清和纯化的抗CSF组分均抑制小鼠粒细胞-巨噬细胞集落形成。纯化的抗体仅含IgG，蛋白质浓度降至血清IgG值的0.1%。这些组分应是研究粒细胞和巨噬细胞分化的有用工具。
