Guihard G, Proteau S, Payet M D, Escande D, Rousseau E
INSER U533, Hôtel-Dieu, Nantes, France.
FEBS Lett. 2000 Jul 7;476(3):234-9. doi: 10.1016/s0014-5793(00)01752-x.
Nuclear ionic channels (NICs) represent ubiquitous structures of living cells, although little is known about their functional properties and encoding genes. To characterize NICs, liver nuclear membrane vesicles were reconstituted into either planar lipid bilayers or proteoliposomes. Reconstitution of nuclear envelope (NE) vesicles into planar lipid bilayer proceeded with low efficiency. NE vesicle reconstitution into proteoliposomes led to NIC observations by the patch-clamp technique. Large conductance, voltage-gated, K(+)-permeant and Cl(-)-permeant NICs were characterized. An 80-105-pS K(+)-permeant NIC with conducting sub-state was also recorded. Our data establish that NICs can be characterized upon reconstitution into giant proteoliposomes and retain biophysical properties consistent with those described for native NICs.
核离子通道（NICs）是活细胞中普遍存在的结构，尽管对其功能特性和编码基因了解甚少。为了表征NICs，将肝核膜囊泡重构成平面脂质双层或蛋白脂质体。核包膜（NE）囊泡重构成平面脂质双层的效率很低。将NE囊泡重构成蛋白脂质体可通过膜片钳技术观察到NICs。对大电导、电压门控、钾离子通透和氯离子通透的NICs进行了表征。还记录到了一种具有传导亚状态的80-105皮安钾离子通透NIC。我们的数据表明，NICs在重构成巨型蛋白脂质体后可以被表征，并保留与天然NICs所描述的一致的生物物理特性。
