Innocenti B, Mazzanti M
Dipartimento di Fisiologia e Biochimica Generali, Università Statale, Milano, Italy.
J Membr Biol. 1993 Jan;131(2):137-42. doi: 10.1007/BF02791322.
The observation that the nuclear envelope outer membrane contains ion channels raises the question of whether these conductances communicate between the cytosol and the nuclear envelope cisternae or between the cytosol and the cytoplasm. Failure to detect large, nonselective holes using the patch-clamp technique has led to the speculation that ion channels and nuclear pores are in fact the same. In this paper we present evidence that the ionic channel, recorded in isolated liver nuclei with the patch-clamp configuration of "nucleus-attached," spans the double membrane of the envelope, providing a direct contact between nucleoplasm and cytoplasm.
核膜外膜含有离子通道这一观察结果引发了一个问题，即这些电导是在细胞质溶胶与核膜池之间，还是在细胞质溶胶与细胞质之间进行通信。使用膜片钳技术未能检测到大的非选择性孔，这引发了一种推测，即离子通道和核孔实际上是相同的。在本文中，我们提供证据表明，在“核附着”膜片钳配置下在分离的肝细胞核中记录到的离子通道跨越核膜的双层膜，在核质和细胞质之间提供了直接接触。
