Sorg B S, McNally K M, Welch A J
Biomedical Engineering Laser Laboratory, University of Texas at Austin, Austin, Texas 78712, USA.
Lasers Surg Med. 2000;27(1):73-81. doi: 10.1002/1096-9101(2000)27:1<73::aid-lsm10>3.0.co;2-7.
The purpose of this study was to determine whether solid material reinforcement of a liquid albumin solder coagulum could improve the cohesive strength of the solder and, thus, the ultimate breaking strength of the incision repair in vitro.
STUDY DESIGN/MATERIALS AND METHODS: A 50%(w/v) bovine serum albumin solder with 0.5 or 2.5 mg/ml indocyanine green (ICG) dye was used to repair an incision in bovine aorta. The solder was coagulated with an 806-nm continuous wave diode laser. A 50-micrometer-thick poly(DL-lactic-co-glycolic acid) film was used to reinforce the solder (the controls had solder but no reinforcement). Acute breaking strengths were measured, and the data were analyzed by Student's t-test.
Observations of the failure modes indicate cohesive strength reinforcement of the test specimens vs. the controls. The 2.5 mg/ml ICG reinforced solder was stronger than the controls without reinforcement (P < 0.05) for all laser powers tested. There was no difference between the test specimens and the controls with 0.5 mg/ml ICG solder for low laser powers, but at higher laser powers, the reinforced solder was stronger than the controls (P < 0.05).
Reinforcement of liquid albumin solders in laser-assisted incision repair seems to have advantages in terms of acute breaking strength over conventional methods that do not reinforce the cohesive strength of the solder.
本研究的目的是确定液体白蛋白焊料凝块的固体材料增强是否能提高焊料的内聚强度，从而提高体外切口修复的极限断裂强度。
研究设计/材料与方法：使用含0.5或2.5mg/ml吲哚菁绿（ICG）染料的50%（w/v）牛血清白蛋白焊料修复牛主动脉切口。用806nm连续波二极管激光使焊料凝固。使用50微米厚的聚（DL-乳酸-乙醇酸）膜增强焊料（对照组有焊料但无增强材料）。测量急性断裂强度，并通过学生t检验分析数据。
对失效模式的观察表明，测试样品的内聚强度相对于对照组有所增强。对于所有测试的激光功率，2.5mg/ml ICG增强的焊料比未增强的对照组更强（P<0.05）。对于低激光功率，0.5mg/ml ICG焊料的测试样品与对照组之间没有差异，但在较高激光功率下，增强的焊料比对照组更强（P<0.05）。
在激光辅助切口修复中，液体白蛋白焊料的增强在急性断裂强度方面似乎比不增强焊料内聚强度的传统方法具有优势。
