Sorg B S, Welch A J
Biomedical Engineering Program, University of Texas at Austin, Austin, Texas 78712, USA.
Lasers Surg Med. 2001;28(4):297-306. doi: 10.1002/lsm.1053.
Previous research introduced the concept of using biodegradable polymer film reinforcement of a liquid albumin solder for improvement of the tensile strength of repaired incisions in vitro. In this study, the effect of creating small pores in the PLGA films on the weld breaking strength is studied. Additionally, the effect of hydration on the strength of the reinforced welds is investigated.
STUDY DESIGN/MATERIALS AND METHODS: A 50%(w/v) bovine serum albumin solder with 0.5 mg/mL Indocyanine Green dye was used to repair an incision in bovine aorta. The solder was coagulated with an 806-nm CW diode laser. A poly(DL-lactic-co-glycolic acid) (PLGA) film was used to reinforce the solder (the controls had solder but no reinforcement). Breaking strengths were measured acutely and after hydration in saline for 1 and 2 days. The data were analyzed by ANOVA (P < 0.05) and multiple comparisons of means were performed using the Newman-Keuls test.
The creation of pores in the PLGA films qualitatively improved the film flexibility without having an apparent adverse effect on the breaking strength, while the actual technique of applying the film and solder had more of an effect. The acute maximum average breaking strengths of some of the film reinforced specimens (114.7 g-134.4 g) were significantly higher (P < 0.05) than the acute maximum average breaking strength of the unreinforced control specimens (68.3 g). Film reinforced specimens were shown to have a statistically significantly higher breaking strength than unreinforced controls after 1- and 2-day hydration.
Reinforcement of liquid albumin solders in laser-assisted incision repair appears to have advantages over conventional methods that do not reinforce the cohesive strength of the solder in terms of acute breaking strength and after immersion in moist environments for short periods of time. Using a film with the solder applied to one surface only may be advantageous over other techniques.
先前的研究引入了使用可生物降解聚合物薄膜增强液态白蛋白焊料以提高体外修复切口抗张强度的概念。在本研究中，研究了在聚乳酸-羟基乙酸共聚物（PLGA）薄膜中制造小孔对焊接断裂强度的影响。此外，还研究了水合作用对增强焊接强度的影响。
研究设计/材料与方法：使用含0.5 mg/mL吲哚菁绿染料的50%（w/v）牛血清白蛋白焊料修复牛主动脉切口。焊料用806 nm连续波二极管激光凝固。使用聚（DL-乳酸-乙醇酸）（PLGA）薄膜增强焊料（对照组有焊料但无增强材料）。在急性情况下以及在盐水中水合1天和2天后测量断裂强度。数据采用方差分析（P<0.05）进行分析，并使用纽曼-基尔斯检验进行均值的多重比较。
在PLGA薄膜中制造小孔在定性上改善了薄膜的柔韧性，而对断裂强度没有明显的不利影响，而应用薄膜和焊料的实际技术影响更大。一些薄膜增强标本的急性最大平均断裂强度（114.7 g - 134.4 g）显著高于（P<0.05）未增强对照标本的急性最大平均断裂强度（68.3 g）。在水合1天和2天后，薄膜增强标本的断裂强度在统计学上显著高于未增强对照标本。
在激光辅助切口修复中，增强液态白蛋白焊料在急性断裂强度和短时间浸入潮湿环境后，似乎比不增强焊料内聚强度的传统方法具有优势。仅在一个表面应用焊料的薄膜可能比其他技术更具优势。
