Sorg Brian S, Welch Ashley J
Biomedical Engineering Program, University of Texas at Austin, 78712, USA.
Lasers Surg Med. 2003;32(3):215-23. doi: 10.1002/lsm.10156.
The purpose of this study was to examine the impact of a polymer film for liquid solder strength reinforcement on the short term healing of a wound closed by laser-tissue soldering.
Full thickness incisions created on the dorsum of Sprague-Dawley rats were closed by laser-tissue soldering: albumin solder with Indocyanine Green (ICG) dye was inserted between the incision edges and photothermally coagulated with a diode laser. A poly(DL-lactic-co-glycolic acid) (PLGA) polymer film was implanted subcutaneously in the bottom of the incision (controls had no film). Specimens were harvested at 0, 3, 7, and 14 days for breaking strength testing and histological analysis.
Breaking strengths of the controls at 0 and 14 days were statistically stronger than the specimens with the implanted films (t-test, P < 0.05). A slight difficulty in apposing the wound edges due to the film presence may have contributed to the low acute strengths. Interference with the wound contraction process by the films possibly contributed to the lower breaking strength at 14 days. Wound histology indicated a mild foreign body reaction to the polymer film material.
The polymer film was well tolerated by the tissue, and the tissue response to the material was consistent with that seen in the literature. The breaking strength differences between control and film-implanted specimens at 0 and 14 days were probably the result of mechanical complications (tissue apposition and wound contraction) due to the presence of the film, and not due to the film material itself. The use of polymer film patches for liquid solder reinforcement and breaking strength enhancement may have certain application specific issues that need to be addressed. Strategies to account for these issues require further research.
本研究旨在探讨一种用于增强液体焊料强度的聚合物薄膜对激光组织焊接闭合伤口短期愈合的影响。
在Sprague-Dawley大鼠背部制作全层切口,通过激光组织焊接进行闭合:将含有吲哚菁绿(ICG)染料的白蛋白焊料置于切口边缘之间,并用二极管激光进行光热凝固。将聚(DL-乳酸-乙醇酸)(PLGA)聚合物薄膜皮下植入切口底部(对照组无薄膜)。在0、3、7和14天采集标本进行抗张强度测试和组织学分析。
对照组在0天和14天的抗张强度在统计学上高于植入薄膜的标本(t检验,P<0.05)。由于薄膜的存在,在对合伤口边缘时稍有困难,这可能导致了较低的急性强度。薄膜对伤口收缩过程的干扰可能是导致14天时抗张强度较低的原因。伤口组织学显示对聚合物薄膜材料有轻度异物反应。
组织对聚合物薄膜耐受性良好,组织对该材料的反应与文献报道一致。对照组与植入薄膜标本在0天和14天的抗张强度差异可能是由于薄膜的存在导致机械并发症(组织对合和伤口收缩),而非薄膜材料本身所致。使用聚合物薄膜贴片增强液体焊料强度和抗张强度可能存在某些特定应用问题需要解决。针对这些问题的策略需要进一步研究。