Salomonsen Charlotte Mark, Boye Mette, Høiby Niels, Jensen Trine H, Hammer Anne Sofie
National Veterinary Institute, Technical University of Denmark, Hangoevej 2, DK-8200 Aarhus N., Denmark (Salomonsen, Jensen, Hammer) and Bülowsvej 27, DK-1870 Frederiksberg C., Denmark (Boye); Department of Clinical Microbiology, Rigshospitalet, Blegdamsvej 9, DK-2100 Copenhagen Ø, Denmark (Høiby).
Can J Vet Res. 2013 Jul;77(3):199-204.
Hemorrhagic pneumonia can be a major cause of mortality in farmed mink in the fall. In its classic form, hemorrhagic pneumonia is caused by the bacterium Pseudomonas aeruginosa. In recent years, however, outbreaks of this type of pneumonia that are associated with hemolytic Escherichia coli have also occurred in farmed mink. The purpose of this study was to compare histological lesions of acute hemorrhagic pneumonia associated with both P. aeruginosa and E. coli in mink, including a description of tissue distribution of pathogens, in an attempt to differentiate between the 2 disease entities based on histopathology. The study included material submitted for diagnostic investigation to the National Veterinary Institute in Denmark from 2006 to 2009. Altogether, 19 cases of hemorrhagic pneumonia with a pure lung culture of P. aeruginosa and 18 cases of hemorrhagic pneumonia with a pure lung culture of E. coli were examined. Formalin-fixed paraffin-embedded lung tissue obtained from the mink was examined by histology and fluorescence in-situ hybridization (FISH). It was possible to detect a slight histological difference between hemorrhagic pneumonia caused by P. aeruginosa and by E. coli, as P. aeruginosa was most often found surrounding blood vessels and lining the alveoli, while E. coli showed a more diffuse distribution in the lung tissue. Furthermore, P. aeruginosa often elicited a very hemorrhagic response in the lung, while infection with E. coli was associated with a higher frequency of alveolar edema and mild lymphoid cuffing in the lungs.
出血性肺炎可能是秋季养殖水貂死亡的主要原因。典型的出血性肺炎由铜绿假单胞菌引起。然而，近年来，养殖水貂中也出现了与溶血性大肠杆菌相关的此类肺炎疫情。本研究的目的是比较水貂中与铜绿假单胞菌和大肠杆菌相关的急性出血性肺炎的组织学病变，包括病原体的组织分布描述，试图基于组织病理学区分这两种疾病实体。该研究纳入了2006年至2009年提交给丹麦国家兽医研究所进行诊断调查的材料。总共检查了19例肺培养物为纯铜绿假单胞菌的出血性肺炎病例和18例肺培养物为纯大肠杆菌的出血性肺炎病例。对从水貂获取的福尔马林固定石蜡包埋肺组织进行组织学检查和荧光原位杂交（FISH）。可以检测到由铜绿假单胞菌和大肠杆菌引起的出血性肺炎之间存在轻微的组织学差异，因为铜绿假单胞菌最常出现在血管周围和肺泡内衬，而大肠杆菌在肺组织中分布更弥散。此外，铜绿假单胞菌常引发肺部非常明显的出血反应，而大肠杆菌感染与肺部肺泡水肿和轻度淋巴样套袖形成的频率较高有关。
