钙离子载体诱导硬壳蛤卵母细胞核破裂和减数分裂

Induction of nuclear breakdown and meiosis in Spisula solidissima oocytes by calcium ionophore.

作者信息

Schuetz A W

出版信息

J Exp Zool. 1975 Mar;191(3):433-40. doi: 10.1002/jez.1401910313.

DOI:10.1002/jez.1401910313

Abstract

Induction of germinal vesicle breakdown and reinitiation of the meiotic process resulted following exposure of immature Spisula solidissima oocytes to divalent ionophore A23187. Meiosis continued to completion in the presence of the ionophore; however, parthenogenetic development of the gametes did not occur. Incubation of oocytes in calcium deficient media (Ca++ free or Ca++Mg++ free seawater) but not Mg++ free seawater inhibited ionophore induced maturation. Effects of calcium deficiency were reversible. The results suggest that nuclear disintegration and the meiotic process induced by sperm are mediated by ionic stimuli and that the establishment of meiotic arrest is linked to the availability of calcium within the oocyte.

摘要

将未成熟的硬壳蛤卵母细胞暴露于二价离子载体A23187后，诱导了生发泡破裂并重新启动减数分裂过程。在离子载体存在的情况下，减数分裂持续完成；然而，配子未发生孤雌生殖发育。在缺钙培养基（无Ca++或无Ca++Mg++的海水）中孵育卵母细胞，但不是在无Mg++的海水中，会抑制离子载体诱导的成熟。缺钙的影响是可逆的。结果表明，精子诱导的核解体和减数分裂过程是由离子刺激介导的，减数分裂停滞的建立与卵母细胞内钙的可用性有关。