通过电泳将钙离子导入非洲爪蟾卵母细胞皮层会触发减数分裂起始。

Electrophoretic introduction of calcium ions into the cortex of Xenopus laevis oocytes triggers meiosis neinitiation.

作者信息

Moreau M, Doree M, Guerrier P

出版信息

J Exp Zool. 1976 Sep;197(3):443-9. doi: 10.1002/jez.1401970318.

DOI:10.1002/jez.1401970318

Abstract

Meiosis reinitiation has been triggered via electrophoretic driving of Ca++ into the cortex of Xenopus laevis oocytes freed from functional follicle cells by collagenase treatment. Mg++ could not be substituted for Ca++. Ca++ induced maturation resembles progesterone induced maturation in so far both are inhibited by cycloheximide, give rise to amplifiable MPF in the cytoplasm of treated oocytes and allow chromosome condensation and the formation of a normal meiotic spindle.

摘要

通过电泳将钙离子驱入经胶原酶处理去除功能性滤泡细胞的非洲爪蟾卵母细胞皮层，从而引发减数分裂重新启动。镁离子不能替代钙离子。钙离子诱导的成熟与孕酮诱导的成熟相似，因为两者都受放线菌酮抑制，在处理过的卵母细胞细胞质中产生可放大的成熟促进因子，并允许染色体凝聚和形成正常的减数分裂纺锤体。