关于六氯酚和铜离子载体诱导的发育中大鼠脊髓损伤中2', 3'-环核苷酸3'-磷酸二酯酶（CNPase）活性的补充研究
Supplemental study on 2', 3'-Cyclic Nucleotide 3'-Phosphodiesterase (CNPase) activity in developing rat spinal cord lesions induced by hexachlorophene and cuprizone.
作者信息
Kanno Takeshi, Kurotaki Tetsuro, Yamada Naoaki, Tomonari Yuki, Sato Junko, Tsuchitani Minoru, Kobayashi Yoshiyasu
机构信息
Pathology Department, Kashima Laboratory, Nonclinical Research Center, LSI Medience Corporation, 14-1 Sunayama, Kamisu, Ibaraki 314-0255, Japan.
School of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
出版信息
J Vet Med Sci. 2019 Oct 10;81(9):1368-1372. doi: 10.1292/jvms.19-0096. Epub 2019 Aug 23.
In a previous study, we showed that 2', 3'-Cyclic Nucleotide 3'-Phosphodiesterase (CNPase) expression is induced in different temporal patterns in the cerebrum, cerebellum and medulla oblongata of hexachlorophene (HCP) and cuprizone (CPZ) treated rats. Here, we additionally examined the histopathological changes and CNPase expression in the spinal cord to clarify the reproducibility of different temporal patterns of CNPase expression in the spinal cord showing low degree or lack of spongy changes. Spongy changes were observed in HCP-treated rats, but not in CPZ-treated rats. Immunohistochemistry showed that intense expression of CNPase was not induced following HCP or CPZ treatment. Our data reveal that expression intensity of CNPase may be dependent on the degree of HCP- and CPZ-induced damage of the myelin sheath.
在之前的一项研究中，我们发现，在六氯酚（HCP）和铜螯合剂（CPZ）处理的大鼠的大脑、小脑和延髓中，2',3'-环核苷酸3'-磷酸二酯酶（CNPase）的表达呈现出不同的时间模式。在此，我们进一步检查了脊髓中的组织病理学变化和CNPase表达，以阐明在脊髓中显示低度或缺乏海绵状变化的CNPase表达不同时间模式的可重复性。在HCP处理的大鼠中观察到了海绵状变化，但在CPZ处理的大鼠中未观察到。免疫组织化学显示，HCP或CPZ处理后未诱导CNPase的强烈表达。我们的数据表明，CNPase的表达强度可能取决于HCP和CPZ诱导的髓鞘损伤程度。
Zotero 插件