Cai S, Liu X, Glasser A, Volberg T, Filla M, Geiger B, Polansky J R, Kaufman P L
Department of Ophthalmology & Visual Sciences, University of Wisconsin, Madison, WI 53792-3220, USA.
Mol Vis. 2000 Jul 28;6:132-43.
Determine the effects of the actin cytoskeleton disrupting compound latrunculin-A (LAT-A) on morphology, cytoskeleton, and cellular adhesions of cultured human trabecular meshwork (HTM) cells.
HTM cells were cultured to high confluence with endothelial-like morphology and treated with LAT-A at different doses and duration. Topography of living cells was evaluated by videomicroscopy. Distribution and organization of the actin-based cytoskeleton, vinculin- and paxillin-containing focal contacts, and beta-catenin-rich intercellular adhesions were determined by immunofluorescence and digital microscopy.
LAT-A induced pronounced but highly reversible rounding of HTM cells, intercellular separation, and disruption of actin filaments. beta-catenin-rich intercellular adherens junctions were particularly sensitive to LAT-A. Vinculin- and paxillin-containing focal contacts were only partially affected and appeared to be more resistant to the drug than the intercellular interactions.
The increase in outflow facility in the living primate eye induced by LAT-A may be due to the disorganization and disruption of the actin cytoskeleton and its associated cellular adhesions in the trabecular meshwork.
确定肌动蛋白细胞骨架破坏化合物拉春库林 - A（LAT - A）对培养的人小梁网（HTM）细胞的形态、细胞骨架和细胞黏附的影响。
将HTM细胞培养至具有内皮样形态的高汇合度，并用不同剂量和持续时间的LAT - A处理。通过视频显微镜评估活细胞的形貌。通过免疫荧光和数字显微镜确定基于肌动蛋白的细胞骨架、含纽蛋白和桩蛋白的黏着斑以及富含β - 连环蛋白的细胞间黏附的分布和组织。
LAT - A诱导HTM细胞明显但高度可逆的变圆、细胞间分离以及肌动蛋白丝的破坏。富含β - 连环蛋白的细胞间黏附连接对LAT - A特别敏感。含纽蛋白和桩蛋白的黏着斑仅受到部分影响，并且似乎比细胞间相互作用对该药物更具抗性。
LAT - A在活体灵长类动物眼中引起的房水流出易度增加可能是由于小梁网中肌动蛋白细胞骨架及其相关细胞黏附的紊乱和破坏。
