通过与纺锤菌素和偏端霉素A相互作用，（dA-dT）依赖性地使DNA模板特性失活。
(dA-dT) dependent inactivation of the DNA template properties by interaction with netropsin and distamycin A.
作者信息
Wähnert U, Zimmer O, Luck G, Pitra O
出版信息
Nucleic Acids Res. 1975 Mar;2(3):391-404. doi: 10.1093/nar/2.3.391.
The inhibitory effect of the polypeptide antibiotics netropsin and distamycin A on DNA dependent nucleic acid synthesis has been shown to be related to the base composition of the template DNA. A number of natural DNA's of quite different dA-dT content as well as poly (dI-dC)-poly (dI-dC), poly (dA-dT)-poly (dA-dT), poly (dA) - poly (dT) and poly (dG) - poly (dC) has been studied as templates in DNA and in part in RNA polymerase reaction. The highest binding efficiency of netropsin existing for (dA-dT) - containing DNA polymers and the less pronounced interaction with the (dI-dC)-containing polymer shown by the melting and CD spectrral behaviour of the complexes are entirely reflected in the template inactivation. The same is evident for distamycin A. However, in contrast to netropsin the antibiotic distamycin A exhibits some binding tendency to poly (dG) - poly (dC). Binding effects of a netropsin derivative to DNA and (dA-dT) -containing polymers suggest the importance of hydrogen bonds of the peptide groups in the complex formation.
多杀菌素和偏端霉素A这两种多肽抗生素对依赖DNA的核酸合成的抑制作用已被证明与模板DNA的碱基组成有关。人们研究了许多dA-dT含量差异很大的天然DNA以及聚（dI-dC）-聚（dI-dC）、聚（dA-dT）-聚（dA-dT）、聚（dA）-聚（dT）和聚（dG）-聚（dC），将其作为DNA以及部分RNA聚合酶反应中的模板。多杀菌素对含（dA-dT）的DNA聚合物具有最高的结合效率，而通过复合物的熔解和圆二色光谱行为显示其与含（dI-dC）聚合物的相互作用较弱，这在模板失活中得到了充分体现。偏端霉素A也是如此。然而，与多杀菌素不同的是，抗生素偏端霉素A对聚（dG）-聚（dC）表现出一定的结合倾向。多杀菌素衍生物与DNA和含（dA-dT）聚合物的结合效应表明肽基团的氢键在复合物形成中具有重要作用。
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